Nur77, PE, clone: 12.14, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 12-5965-80
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: This 12.14 monoclonal antibody reacts with mouse Nur77 (also known as NR4A1, TR3, NGFI-B, or NAK1), an inducible orphan nuclear receptor. Expressed in thymocytes and T cell lines, Nur77 promotes apoptosis and plays a role in thymocyte negative selection. Additionally, Nur77 has been shown to be critical for steroid biosynthesis in Leydig cells as well as for the effects of dopamine. In addition, Nur77 has been shown to interact with FoxP3 in regulatory T cells. However, our results with this antibody do not correlate with this observation.Applications Reported: This 12.14 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 12.14 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One-step protocol for intracellular (nuclear) proteins. This antibody can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
|Flow Cytometry (Intracellular Staining)|
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
|NGFI-B, NR4A1, TR3, NAK1|
For Research Use Only.