Macherey-Nagel™ NucleoSpin™ RNA Plant and Fungi

Universal kit designed to isolate high-integrity RNA from challenging plant and fungal samples using silica membrane mini spin columns, for use in a variety of downstream applications. Kits with 10, 50 or 250 preps available.

Brand:  Macherey-Nagel™ 740120.250

Product Code. 15865244

Description

Plant and fungal material is diverse in terms of structure, cell wall composition, biochemical compounds and the types of organs. Common products for RNA isolation often fails to efficiently purify RNA from challenging plant samples.

• Tailored protocols for diverse starting materials (e.g. seeds, roots, stems, needles)
• Convenient handling and efficient isolation of high integrity RNA
• NucleoSpin RNA Plant and Fungi Filters for lysate clearing included
• Designed for samples rich in secondary metabolites, sugar, starch or other compounds that interfere with nucleic acid purification methods
• Can also be used for standard samples, such as Arabidopsis leaves
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays

Specifications

• Format: Mini Spin Column
• Elution Volume: 50 μL
• Efficiency: Binding capacity: 200 μg
• For Use With (Application): Isolation of RNA from plant and fungal samples
• No. of Reactions: 250
• Sample Size: < 500 mg
• Test Time: 25 min / 6 preps (preparation time)

• Isolation Technology: Silica Membrane
• Sample Type: Plant or Fungal Material (Seeds, Roots, Stems, Needles)
• Final Product Type: High integrity RNA
• Includes: Filters, Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), buffers, RNase free water
• Purity: A₂₆₀/A₂₈₀: 1.9 - 2.1
• Test Sensitivity: RNA integrity number (RIN): 7 - 9
• Yield: 20 - 70 μg

Safety and Handling

Product Identifier

• NucleoSpin RNA Plant and Fungi

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• Skin corrosion/irritation Category 2
• Serious eye damage/eye irritation Category 2

Hazard Statement

• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.

Precautionary Statement

• P264-Wash with water thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P330-Rinse mouth.

Supplemental information

• MIXTURE LIST-Contains : guanidine hydrochloride, ethylendinitrilo tetraacetic acid (EDTA)