Invitrogen™ Rat IL-1 beta ELISA Kits

Measure levels of rat interleukin-1 beta (IL-1b) in rat serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples using 96-well plates.

Brand:  Invitrogen™ ER2IL1B

Product Code. 10591674

Description

Assay IL-1β (rat interleukin-1 beta) levels in rat serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Rat IL-1 beta ELISA Kits.

These rat IL-1 beta ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 beta (IL-1β) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

• Target: Natural and recombinant rat interleukin-1 beta (IL-1β)

• Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

• Measurement: Absorbance at 450nm minus absorbance at 550nm

• Assay Range: 25.6 to 2500pg/mL

• Sensitivity: < 12pg/mL

• Precision: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%

• Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

• Total Assay Time: 3.5 hours

• Sample Size: 50μL

• Specificity: No significant cross-reaction with rat IFNγ, IL-1α, IL-2, IL-4, IL-6, IL-10, MCP-1, MIP-1α, or RANTES, or human, mouse or pig IL-1β

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Specifications

• Assay Range: 25.6 to 2500pg/mL
• Description: Rat IL-1 beta ELISA Kit
• Sample Type: Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum
• Sufficient For: 96 microplate wells
• Measurement: Absorbance at 450nm minus absorbance at 550nm
• Quantity: 1 Plate Kit
• Target: Natural and recombinant rat interleukin-1 beta (IL-1ß)

• Assay Sensitivity: <12pg/mL
• Immunoassay Kit Format: Colorimetric ELISA (TMB substrate) with Plates
• Specificity: No significant cross-reaction with rat IFNg, IL-1a, IL-2, IL-4, IL-6, IL-10, MCP-1, MIP-1a, or RANTES, or human, mouse or pig IL-1ß
• Kit Contents: Lyophilized Recombinant Rat IL-1 beta Standard, 2 vials, TMB Substrate Solution, 12mL, Streptavidin-HRP Concentrate, 75µL, Stop Solution, 13mL, Sample Diluent, 25mL, HRP Dilution Buffer, 13mL, Biotinylated Antibody Reagent, 13mL, Anti-Rat IL-1 beta Precoated 96-well Strip Plate, 1 ea, Adhesive Plate Covers, 6 ea, 30X Wash Buffer, 50mL
• Precision: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
• Sample Volume: 50 μL
• Total Assay Time: 3.5 hr.