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Invitrogen™ Rat IL-1 beta ELISA Kits

Measure levels of rat interleukin-1 beta (IL-1b) in rat serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples using 96-well plates.

Brand:  Invitrogen™ ER2IL1B2

Code : PN

Additional Details : Weight : 0.10000kg

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Product Code. 10501684

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance


Description

Description

These rat IL-1 beta ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 beta (IL-1β) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant rat interleukin-1 beta (IL-1β)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 25.6 to 2500pg/mL

  • Sensitivity: < 12pg/mL

  • Precision: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3.5 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with rat IFNγ, IL-1α, IL-2, IL-4, IL-6, IL-10, MCP-1, MIP-1α, or RANTES, or human, mouse or pig IL-1β

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Specifications

Specifications

NP_113700.2, NM_031512.2
Rat IL-1 beta ELISA Kit
Complete ELISA kit with coated 96-well plates
24494
Streptavidin-HRP Concentrate, 2 x 75µL, 30X Wash Buffer, 2 x 50mL, Adhesive Plate Covers, 12 ea, Anti-Rat IL-1 beta Precoated 96-well Strip Plate, 2 ea, Biotinylated Antibody Reagent, 2 x 13mL, HRP Dilution Buffer, 2 x 13mL, Lyophilized Recombinant Rat IL-1 beta Standard, 4 vials, Stop Solution, 2 x 13mL, TMB Substrate Solution, 2 x 12mL, Sample Diluent, 2 x 25mL
Absorbance at 450nm minus absorbance at 550nm
<12pg/mL
50µL
2 x 96 microplate wells
Natural and recombinant rat interleukin-1 beta (IL-1ß)
25.6 to 2500pg/mL
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Chromogenic Detection
Anti-Rat IL-1 beta Precoated 96-well Strip Plate, 2 each; Lyophilized Recombinant Rat IL-1 beta Standard, 4 vials; Sample Diluent, 2 x 25mL; Biotinylated Antibody Reagent, 2 x 13mL; 30X Wash Buffer, 2 x 50mL; Streptavidin-HRP Concentrate, 2 x 75μL; HRP Dilution Buffer, 2 x 13mL; TMB Substrate Solution, 2 x 12mL; Stop Solution, 2 x 13mL; Adhesive Plate Covers, 12 each
Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
2 plate kit
Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
No significant cross-reaction with rat IFNg, IL-1a, IL-2, IL-4, IL-6, IL-10, MCP-1, MIP-1a, or RANTES, or human, mouse or pig IL-1ß
3.5 hr.
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