Invitrogen™ alpha Synuclein Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ KHB0061

Product Code. 10582825

Includes: Pre-coated plate, standard, detector antibody, anti-IgG-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

Description

The Human alpha Synuclein ELISA quantitates Hu alpha Synuclein in human cell and tissue lysates. The assay will exclusively recognize both natural and recombinant Hu alpha Synuclein. Principle of the method The Human alpha Synuclein solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples,standards,or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody,a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.

Alpha-Synuclein belongs to the Synuclein family, which includes beta and gamma Synuclein, and is predominantly expressed in neurons, and concentrated at synaptic terminals. Synucleins may be involved in the regulation of dopamine release and transport and the modulation of synaptic vesicle function. Alpha-Synuclein is a small, presynaptic protein that is the major nonamyloid component of the pathological inclusions characteristic of a wide range of neurodegenerative disorders, collectively known as synucleinopathies. Alpha-Synuclein is involved in the formation of SNARE complexes, and most significantly, aggregated alpha-synuclein is one of the major components found in the Lewy bodies that occur in Parkinson's disease (PD) and other neurodegenerative disorders. Mutation of the alpha-synuclein gene is associated with familial forms of PD. Alpha-Synuclein is known to reduce the fibrillization of the microtubule-associated protein, tau, to bind various targets such as 14-3-3, protein kinase C, synphilin-1, Elk and Tat-binding protein 1. The alpha and beta synucleins are found primarily in brain tissue where they are seen mainly in neuronal cytosol and presynaptic terminals. The antibody specific for alpha-synuclein does not cross react with beta-synuclein and will be useful for the confirmation of Parkinson's disease and Lewy body dementia and other studies for the localization and detection of alpha-synuclein. Four alternatively spliced transcripts that encode two different isoforms of the alpha-synuclein protein have been identified.

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Shipping condition: Wet ice

Specifications

• Accession Number: P37840
• Assay Sensitivity: 0.2 ng/mL
• Conjugate: Biotin
• Sample Type: Cell Lysates
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: NACP,PARK1,PARK4,PD1
• Interassay CV: 6.4%
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Marker Gene: α-Synuclein
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.

• Assay Range: 0.23-15 ng/mL
• Concentration: 1/56 to 100ng/mL, 1.56 to 100ng/mL
• Product Type: ELISA Kit
• Target Species: Human
• Gene ID (Entrez): 6622
• Incubation Time: 4 hr.
• Intraassay CV: 5.7%
• Label or Dye: HRP
• Quantity: 96 Tests
• Sample Volume: Cell Lysate,10 μL
• Target: Human
• Total Assay Time: 4 hr.

For Research Use Only. Not for use in diagnostic procedures.