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Invitrogen™ MIP-1a Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ KAC2201

318.01 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : 50

Additional Details : Weight : 0.50000kg

Product Code. 10776513

  • £477.00 / 96 tests
Estimated Shipment: 22-04-2024
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For Research Use Only. All usage must comply with product instructions.
 

Includes: Pre-coated plate, standard, controls, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

Description

Description

The Human Macrophage Inflammatory Protein-1 alpha/Chemokine Ligand 3 (Hu MIP-1α/CCL3) ELISA quantitates Hu MIP-1α in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MIP-1α. Principle of the method The Human MIP-1αsolid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CCL3 (MIP1a, Macrophage Inflammatory Protein-1 alpha, MIP-1 alpha) has a molecular weight of approximately 8 kDa, belongs to the family of chemotactic cytokines, and is involved in chemotactic and proinflammatory effects, and homeostasis. CCL3 is produced by lymphocytes, macrophages and dendritic cells, and is involved in inflammatory response of blood monocytes and tissue macrophages. Also, CCL3 can exist as a naturally occurring heterodimer with MIP-1beta and has been shown to have antiviral activity against HSV-1. CCL3 protein can bind with high affinity to the CCR1 and CCR5 receptors.

Order Info

Shipping Condition: Wet ice

Specifications

Specifications

P10147
<2 pg/mL
ELISA Kit
Human
Colorimetric Microplate Reader
G0S19-1,LD78ALPHA,MIP-1-alpha,MIP1A,SCYA3
6.5%
HRP
96 Tests
Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
Human
3 hr.
15.6-1000 pg/mL
Biotin
Plasma, Serum, Supernatant
Colorimetric
6348
7.1%
Pre-coated 96 well plate, Controls, Adhesive plate covers, Incubation Buffer, Standard Dilution Buffer, Strepavidin-HRP conjugate, Biotinylated Detection Antibody, Wash Buffer, Chromogen, Wash Buffer, Chromogen, Stop Solution
<2pg/mL
RUO
2°C to 8°C
1 hr. 20 min.
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For Research Use Only. Not for use in diagnostic procedures.