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Invitrogen™ IL-8 Porcine ELISA Kit

Cytokine chemoattractant protein for neutrophils

Brand:  Invitrogen™ KSC0081

318.01 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : 50

Additional Details : Weight : 0.70000kg

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Product Code. 10503215

  • £477.00 / Each
Estimated Shipment: 22-04-2024
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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

The Porcine Interleukin 8 (Por IL-8) ELISA quantitates Por IL-8 in porcine serum, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Por IL-8. Principle of the method The Porcine IL-8 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL-8 (Interleukin-8) is a 72 amino acid pro-inflammatory CXC chemokine designated CXCL8, secreted by endothelial cells and monocytes, which acts as a major chemoattractant for neutrophils and also T cells and basophils. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C.
Specifications

Specifications

P26894
<10 pg/mL
ELISA Kit
Porcine
Colorimetric Microplate Reader
GCP-1,GCP1,IL8,LECT,LUCT,LYNAP,MDNCF,MONAP,NAF,NAP-1,NAP1
7.1%
HRP
96 Tests
Serum, 50 μL; Supernatant, 100 μL
Porcine
4 hr.
31.2-2000 pg/mL
Biotin
Serum, Supernatant
Colorimetric
396880
8.6%
Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
<10pg/mL
RUO
2°C to 8°C
1 hr. 20 min.
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For Research Use Only. Not for use in diagnostic procedures.