Invitrogen™ IL-12 p40 Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ KAC1561

Product Code. 10344013

Includes: Pre-coated plate, standard, controls, antibody-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

Description

The Human Interleukin-12 p40(Hu IL-12 p40) ELISA quantitates Hu IL-12 p40 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-12 p40. Principle of the method The Human IL-12 p40 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Recognizes natural and recombinant human IL-12p40.

Order Info

Shipping Condition: Wet ice

Specifications

• Accession Number: P29460
• Assay Sensitivity: 1.5 pg/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CLMF,CLMF2,IL-12B,IMD28,IMD29,NKSF,NKSF2
• Intraassay CV: 4%
• Label or Dye: HRP
• Quantity: 96 Tests
• Sample Volume: Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL
• Target: Human
• Total Assay Time: 4 hr. 30 min.

• Assay Range: 15-1300 pg/mL
• Conjugate: HRP
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): Colorimetric
• Gene ID (Entrez): 3593
• Interassay CV: 6.7%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample diluent, Incubation Buffer, HRP conjugated Detection Antibody, Conjugate Diluent, Wash Buffer, Chromogen, Stop Solution, Controls
• Precision: 1.5pg/mL
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.

For Research Use Only. Not for use in diagnostic procedures.