High selectivity for poly-histidine tags results in extremely low background levels. Bead surface chemistry is cobalt-based and offers exceptionally fast binding kinetics and a high yield per microliter of beads. The entire protocol (from cleared lysate to purified protein) takes only 20 minutes. Protocol includes instructions for making the required buffers and methods for optimizing buffer composition. Dynabeads His-Tag Isolation and Pulldown replaces Dynabeads TALON™. Dynabeads His-Tag Isolation and Pulldown has a optimized binding chemistry that offers significant advantages over Dynabeads TALON which were coated in the TALON chemistry. This optimization results in a cleaner prep with higher yield of his-tag protein and extremely low background levels. Dynabeads His-Tag Isolation and Pulldown is designed for isolating His-tagged proteins from cleared lysates. Isolated his-tagged proteins can either be eluted from the beads or kept on the beads for use in bead-based downstream assays. Bead bound his-tagged proteins can be used in various downstream assays such as bait-prey pulldowns, etc.
Technology used for discontinued Dynabeads TALON was licensed from Clontech. TALON is a registered trademark of Clontech Laboratories Inc., USA.
His-Tagged Protein Purification, Protein and Peptide Purification, Protein Complex Pull-Down, Protein Sample Preparation and Protein Purification, Proteins, Expression, Isolation and Analysis