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Invitrogen™ Dynabeads™ His-Tag Isolation and Pulldown
Description
Includes
Protocols. Dynabeads™ His-Tag Isolation and Pulldown beads are supplied as a suspension supplied in 20% ethanol.
Requires
Buffers
Dynabeads™ His-Tag Isolation and Pulldown beads are 1-μm monosized, monodispersed, superparamagnetic beads with an NTA-Cobalt-based ligand which has a high affinity towards His-tags and similar structures on molecular targets. Their small but uniform bead size presents a high surface area per mg beads and a correspondingly high capacity for the target molecule. They enable single-step isolation of tagged molecules in manual or automated workflows that helps ensure good recovery of the target. Dynabeads His-Tag Isolation and Pulldown beads can also be a great starting point for other advanced experimental approaches where solid magnetic support is needed.
Benefits of Dynabeads His-Tag and Pulldown beads include:
• Performance—high sensitivity and reproducibility securing consistent results
• Efficiency—fast binding kinetics and high binding capacity
• Flexibility—flexible protocols with gentle and efficient liquid-phase reaction kinetics
• Automation-ready—automation protocols available with the KingFisher Purification instruments
Good signal-to-noise for numerous applications
Dynabeads His-Tag Isolation and Pulldown beads are an ideal tool for isolation of tagged recombinant proteins. They are also suited for other applications with high signal-to-noise requirements. Using the principles of immobilized metal affinity binding together with the inert surface of Dynabeads magnetic beads helps ensure a clean capture with extremely low carryover of unwanted proteins. Since the bead surface is loaded with cobalt ions, it is also possible to make a stable and lasting bond with the target molecule through oxidation if the downstream applications require it. The beads are added to the solution containing the target and mixed for 5-10 minutes. After washing, the target is eluted off and ready for downstream applications.
More than just a pulldown
In addition to being a great tool for protein pulldown and isolation, the Dynabeads His-Tag Isolation and Pulldown beads can serve as a platform for target immobilization. The bead carries cobalt Co2+ ions, so it is possible to cement the bond between the bead and the target just by using mildly oxidative conditions, such as 10 mM hydrogen peroxide for 30–60 minutes. This oxidizes cobalt to the Co3+ state, making the connection between the bead and the target permanent. It will not elute in the commonly used elution buffers for IMAC. This can later be used for applications requiring a strongly bound target, without the risk of reduced target yield.
Automation-ready for use with KingFisher purification systems
The 1-μm magnetic beads are ideal for high-throughput enrichment. This process can be automated using any of our KingFisher sample purification systems or liquid handlers.
Commercial supply
Our manufacturing sites are ISO 13485-certified. Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility making them an ideal choice for commercial supply. If you are in the process of customizing Dynabeads His-Tag Isolation and Pulldown beads in a commercial product or service or need a larger bulk volume of this product, please contact us at oemdynal@lifetech.com or see our Dynabeads OEM page.
Learn more about Dynabeads magnetic beads and KingFisher Sample Purification Systems:
• See our immunoprecipitation selection table, data, and references
• Dynabeads magnetic beads selection guide
• See magnets for Dynabeads magnetic beads separations
• KingFisher automation protocols
• Watch a video about the KingFisher Flex instrument
Order Info
Shipping Condition: Room Temperature
Specifications
Specifications
| Binding Property | 1.6 mg of a 28 kDa histidine-tagged protein/mL (40 mg) beads |
| Concentration | 40 mg/mL |
| Material | Magnetic Polystyrene |
| Content And Storage | Dynabeads His-Tag Isolation and Pulldown beads are supplied in 20% ethanol. Store at 2°C to 8°C. |
| Diameter (Metric) | 1 μm |
| For Use With (Equipment) | KingFisher Sample Purification System, DynaMag magnets |
| High-throughput Compatibility | Yes |
| Shipping Condition | Ambient temperature |
| Sample Type | Cell Cultures, Tissue Digests, Intact Proteins, Cell Lysates, Cell Extracts |
| Surface Functionality | Cobalt-based immobilized metal affinity chromatography (IMAC) |
| Show More |
For Research Use Only. Not for use in diagnostic procedures.
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