Invitrogen™ Amyloid beta (Aggregated) Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ KHB3491

Product Code. 10766743

Includes: Pre-coated plate, standard, detector antibody, anti-IgG-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

Description

The Human Aggregated Amyloid Beta ELISA quantitates Hu aggregated Aβ in human tissue homogenates, ventricular fluid, CSF, tissue culture supernatant, and buffered solution. The assay will exclusively recognize both natural and recombinant Hu aggregated Aβ. Principle of the method The Human aggregated Aβ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Beta-amyloid is a 40–43 amino acid peptide cleaved from amyloid precursor protein, and a putative gamma-secretase. Amyloid beta (1-40) together with Amyloid beta (1-42) are two major C-terminal variants of the amyloid beta protein, constituting the majority of amyloid beta proteins. Both the amyloid beta (40) and amyloid beta (42) alloforms are major components of the extracellular plaques found in Alzheimer's disease brain tissue.

Order Info

Shipping condition: Wet ice

Specifications

• Accession Number: P05067
• Assay Sensitivity: <0.01 ng/mL
• Conjugate: Biotin
• Sample Type: Cerebrospinal Fluid, Supernatant, Tissue Homogenates, Ventricular Fluid
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: AAA,ABETA,ABPP,AD1I,CTFgamma,CVAP,PN-II,PN2,APP
• Interassay CV: 7.4%
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Marker Gene: Aggregated Aβ
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.

• Assay Range: 0.09-5.7 ng/mL
• Concentration: 0.09 to 5.7ng/mL
• Product Type: ELISA Kit
• Target Species: Human
• Gene ID (Entrez): 351
• Incubation Time: 4 hr.
• Intraassay CV: 7.4%
• Label or Dye: HRP
• Quantity: 96 Tests
• Sample Volume: Cerebrospinal Fluid,100 μL; Supernatant, 50 μL; Tissue Homogenate,50 μL; Ventricular Fluid,100 μL
• Target: Human
• Total Assay Time: 4 hr.

For Research Use Only. Not for use in diagnostic procedures.