Notch2 Rat anti-Human, Mouse, PE, Clone: 16F11, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 12-5786-82

100UG ANTI-HUMAN/MOUSE NOTCH2 PE

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15268829

Quantity Price
1 £ 201.0 / 100µg
Estimated Shipment date
from Supplier 08-12-2016
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Description and Specification

Specification

Antigen Notch2
Applications Flow Cytometry
Clone 16F11
Concentration 0.2mg/mL
Conjugate PE
Format Conjugated
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Host Species Rat
Isotype IgG1
Quantity 100μg
Regulatory Status RUO
Species Reactivity Human, Mouse
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal

This 16F11 monoclonal antibody reacts with the extracellular domain of human and mouse Notch2, one of four members of the Notch family of receptors. Notch receptors are 300kDa single-pass transmembrane proteins. While the extracellular domain contains numerous epidermal growth factor-like repeats for ligand binding, the intracellular domain is involved in cell signaling. Upon binding its membrane-bound ligand (either Delta or Jagged), the Notch receptor undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by γ-secretase. The second cleavage event releases the Notch intracellular domain (NICD), which subsequently translocates into the nucleus, heterodimerizes with the DNA-binding protein RBP-J, recruits co-activator molecules, and ultimately activates transcription.

Notch2 is expressed on thymocytes (i.e., DN1 and DN2 subsets), activated peripheral T cells, and bone marrow. Moreover, expression of Notch2 has been reported to be altered in various cancers and lymphomas. In addition to its role in stem cell hematopoiesis, Notch2 plays a pivotal role in marginal zone B cell development. Notch2 also interacts with phosphorylated CREB1 to induce granzyme B transcription in cytotoxic T cells.