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NOTCH2, PE, clone: 16F11, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-5786-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15268829

  • £174.50 / 100µg


Estimated Shipment date
from Supplier 30-05-2018
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Description

Description

Description: This 16F11 monoclonal antibody reacts with the extracellular domain of human and mouse Notch2, one of four members of the Notch family of receptors. Notch receptors are 300-kDa single-pass transmembrane proteins. While the extracellular domain contains numerous epidermal growth factor-like repeats for ligand binding, the intracellular domain is involved in cell signaling. Upon binding its membrane-bound ligand (either Delta or Jagged), the Notch receptor undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase. The second cleavage event releases the Notch intracellular domain (NICD), which subsequently translocates into the nucleus, heterodimerizes with the DNA-binding protein RBP-J, recruits co-activator molecules, and ultimately activates transcription.Notch2 is expressed on thymocytes (i.e., DN1 and DN2 subsets), activated peripheral T cells, and bone marrow. Moreover, expression of Notch2 has been reported to be altered in various cancers and lymphomas. In addition to its role in stem cell hematopoiesis, Notch2 plays a pivotal role in marginal zone B cell development. Notch2 also interacts with phosphorylated CREB1 to induce granzyme B transcription in cytotoxic T cells.Applications Reported: This 16F11 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 16F11 antibody has been tested by flow cytometric analysis of 24-hour anti-CD3-stimulated mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Notch2
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
O35516, Q04721
AGS2, HJCYS, hN2, Notch homolog 2, neurogenic locus notch homolog protein 2
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
16F11
PE
NOTCH2
Conjugated
Notch2
IgG1, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
4853
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