Notch1 Rat anti-Mouse, PE, Clone: 22E5, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 12-5765-82

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15258819

Quantity Price
1 £ 158.36 / 100µg
Estimated Shipment date
from Supplier 09-05-2017
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Description and Specification

Specification

Applications Flow Cytometry
Quantity 100μg
Isotype IgG2a
Antigen Notch1
Monoclonal or Polyclonal Monoclonal
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gene Alias Notch 1, TAN1, Neurogenic locus notch homolog protein 1
Host Species Rat
Regulatory Status RUO
Conjugate PE
Species Reactivity Mouse
Concentration 0.2mg/mL
Primary or Secondary Primary
Clone 2.20E+06
Format Conjugated

This 22E5 monoclonal antibody reacts with the extracellular domain of mouse Notch1, one of four members of the Notch family of receptors. Notch receptors are 300kDa single-pass transmembrane proteins. While the extracellular domain contains numerous epidermal growth factor-like repeats for ligand binding, the intracellular domain is involved in cell signaling. Upon binding its membrane-bound ligand (either Delta or Jagged), the Notch receptor undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase. The second cleavage event releases the Notch intracellular domain (NICD), which subsequently translocates into the nucleus, heterodimerizes with the DNA-binding protein RBP-J, recruits co-activator molecules, and ultimately activates transcription.

Notch 1 is expressed on thymocytes, bone marrow hematopoietic stem cells, T and NK cells. Lower Notch1 expression levels can be found on B cells and monocytes. Studies show that some subsets of T-cell acute lymphoblastic leukemia (T-ALL) arise due to Notch1 chromosomal translocation with TCRbeta, which results in the expression of constitutively active Notch1. This cell surface receptor is involved in T cell lineage commitment, thymocyte development, and Th2 differentiation.