Notch1 Rat anti-Mouse, APC, Clone: 22E5, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 17-5765-82

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15320750

Quantity Price
1 £ 152.25 / 100µg
Estimated Shipment date
from Supplier 08-05-2017
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Description and Specification

Specification

Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Concentration 0.2mg/mL
Isotype IgG2a
Format Conjugated
Primary or Secondary Primary
Regulatory Status RUO
Host Species Rat
Quantity 100μg
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Antigen Notch1
Applications Flow Cytometry
Clone 2.20E+06
Conjugate APC
Species Reactivity Mouse
Monoclonal or Polyclonal Monoclonal

This 22E5 monoclonal antibody reacts with the extracellular domain of mouse Notch1, one of four members of the Notch family of receptors. Notch receptors are 300kDa single-pass transmembrane proteins. While the extracellular domain contains numerous epidermal growth factor-like repeats for ligand binding, the intracellular domain is involved in cell signaling. Upon binding its membrane-bound ligand (either Delta or Jagged), the Notch receptor undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by γ-secretase. The second cleavage event releases the Notch intracellular domain (NICD), which subsequently translocates into the nucleus, heterodimerizes with the DNA-binding protein RBP-J, recruits co-activator molecules, and ultimately activates transcription.

Notch 1 is expressed on thymocytes, bone marrow hematopoietic stem cells, T and NK cells. Lower Notch1 expression levels can be found on B cells and monocytes. Studies show that some subsets of T-cell acute lymphoblastic leukemia (T-ALL) arise due to Notch1 chromosomal translocation with TCRβ, which results in the expression of constitutively active Notch1. This cell surface receptor is involved in T cell lineage commitment, thymocyte development, and Th2 differentiation.