Notch1 Rat anti-Mouse, APC, Clone: 22E5, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 17-5765-82

100UG ANTI-MOUSE NOTCH1 APC

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15320750

Quantity Price
1 £ 182.0 / 100µg
Estimated Shipment date
from Supplier 27-02-2017
Add to basket


Description and Specification

Specification

Antigen Notch1
Format Conjugated
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Host Species Rat
Applications Flow Cytometry
Isotype IgG2a
Quantity 100μg
Regulatory Status RUO
Species Reactivity Mouse
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal
Clone 2.20E+06
Concentration 0.2mg/mL
Conjugate APC

This 22E5 monoclonal antibody reacts with the extracellular domain of mouse Notch1, one of four members of the Notch family of receptors. Notch receptors are 300kDa single-pass transmembrane proteins. While the extracellular domain contains numerous epidermal growth factor-like repeats for ligand binding, the intracellular domain is involved in cell signaling. Upon binding its membrane-bound ligand (either Delta or Jagged), the Notch receptor undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by γ-secretase. The second cleavage event releases the Notch intracellular domain (NICD), which subsequently translocates into the nucleus, heterodimerizes with the DNA-binding protein RBP-J, recruits co-activator molecules, and ultimately activates transcription.

Notch 1 is expressed on thymocytes, bone marrow hematopoietic stem cells, T and NK cells. Lower Notch1 expression levels can be found on B cells and monocytes. Studies show that some subsets of T-cell acute lymphoblastic leukemia (T-ALL) arise due to Notch1 chromosomal translocation with TCRβ, which results in the expression of constitutively active Notch1. This cell surface receptor is involved in T cell lineage commitment, thymocyte development, and Th2 differentiation.