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NOTCH1, APC, clone: 22E6, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 17-5765-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15320750

  • £156.06 / 100µg


Estimated Shipment
26-07-2018
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Description

Description

Description: This 22E5 monoclonal antibody reacts with the extracellular domain of mouse Notch1, one of four members of the Notch family of receptors. Notch receptors are 300-kDa single-pass transmembrane proteins. While the extracellular domain contains numerous epidermal growth factor-like repeats for ligand binding, the intracellular domain is involved in cell signaling. Upon binding its membrane-bound ligand (either Delta or Jagged), the Notch receptor undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase. The second cleavage event releases the Notch intracellular domain (NICD), which subsequently translocates into the nucleus, heterodimerizes with the DNA-binding protein RBP-J, recruits co-activator molecules, and ultimately activates transcription.Notch 1 is expressed on thymocytes, bone marrow hematopoietic stem cells, T and NK cells. Lower Notch1 expression levels can be found on B cells and monocytes. Studies show that some subsets of T-cell acute lymphoblastic leukemia (T-ALL) arise due to Notch1 chromosomal translocation with TCRbeta, which results in the expression of constitutively active Notch1. This cell surface receptor is involved in T cell lineage commitment, thymocyte development, and Th2 differentiation.Applications Reported: This 22E5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 22E5 antibody has been tested by flow cytometric analysis of 24-hour CD3-stimulated mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Notch1
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q01705
9930111A19Rik, Drosophila Notch homolog 1 (controlling the the ectodermal and neural cell fate in Drosophila), lin-12, major type A protein, Mis6, Motch A, mT14, N1, neurogenic locus notch homolog protein 1, Notch gene homolog 1, Notch homolog 1, translocation-associated, p300, RP23-306D20.12-001, Tan1, translocation-associated notch protein TAN-1, transmembrane receptor Notch1
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
2.20E+06
APC
Notch1
Conjugated
Notch1
IgG2a, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
18128
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