iNOS, clone: CXNFT, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-5920-80
Code : NEW
Additional Details : Weight : 0.50000kg
DescriptionDescription: This CXNFT monoclonal antibody reacts to mouse NOS2 (inducible NOS, iNOS). Nitric oxide synthase enzymes catalyze the formation of nitric oxide from L-arginine through an NADPH- and oxygen-dependent mechanism. There are three isoforms of NOS that are encoded by three separate genes. NOS1 (neuronal NOS, nNOS) and NOS3 (endothelial NOS, eNOS) are constitutively expressed, while NOS2 is induced in response to bacterial endotoxins and inflammatory cytokines such as IFN gamma and TNF alpha. NOS2 is expressed by myeloid-derived suppressor cells and M1 macrophages but not alternatively activated M2 macrophages. NOS enzymes are functionally active only when they form homodimers, and dimerization of NOS2 occurs at steady-state concentrations of free Ca2+ such that NOS2 is functionally active when it is produced.Applications Reported: This CXNFT antibody has been reported for use in intracellular staining followed by flow cytometric analysis, and western blotting. (Fluorochrome conjugated CXNFT is recommended for use in intracellular flow cytometry.).Applications Tested: This CXNFT antibody has been tested by immunoblot analysis of LPS-stimulated mouse thioglycolate-elicited peritoneal exudate cells or by intracellular staining and flow cytometric analysis of mouse NOS2-transiently transfected HEK-293T cells. For immunoblot analysis this can be used at 5 μg/mL. For intracellular staining this can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|Inducible Nitric Oxide Synthase, NOS Type II|
|Flow CytometryþWestern Blot|
|Store at 2-8°C.|
For Research Use Only.