Thermo Scientific™ North2South™ Chemiluminescent Hybridization and Detection Kit

Acquire consistent, highly sensitive Southern blot results with a novel ECL HRP substrate and optimized hybridization and blocking buffers.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 17295

NORTH2SOUTH CHEMI FOR HRP 100ML

UNSPSC: 41105326

Code: PN

Additional Details:
Additional Details: CAS Number: 636-28-2 Weight: 0.80000kg



Product Code. 11876704

Quantity Price
1 £ 243.0 / 100mL
Estimated Shipment date
from Supplier 09-03-2017
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Description and Specification

Specification

Formulation Proprietary luminol and peroxide solutions
Product Name North2South Chemiluminescent Substrate for HRP
Product Type North2South Chemiluminescent Substrate for HRP
Sufficient For

1000cm2 of membrane (10 mini-gel blots)

Quantity 100mL kit
For Use With (Application) Non-radioactive Southern and Northern blotting
Includes Luminol/Enhancer, 50mL; Stable Peroxide Buffer, 50mL
Quantity 100mL kit
Item Description Chemiluminescent Substrate for HRP

The Thermo Scientific North2South Hybridization and Detection Kit combines a novel enhanced luminol substrate for horseradish peroxidase (HRP) with optimized pre-made hybridization and blocking buffers to ensure consistent results and high sensitivity. Any nucleic acid blot can be hybridized with the appropriate biotinylated (biotin-labeled) oligonucleotide probe. After a brief membrane blocking step, the biotin label is detected with streptavidin-HRP and a 5-minute incubation with the chemiluminescent substrate. Results are easily recorded by brief exposure to X-ray film (1-15 minutes) or CCD camera.

Highlights:

  • Sensitivity equal to or greater than 32P
  • Amenable to many formats
  • High level of light output and consistent low background allows for very short exposure times using cooled CCD camera systems or film
  • Ready-to-use buffers and short process time makes switching from radioactive detection to chemiluminescent detection an easy transition, even for the novice user

Documentation

Technical documents