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NK1.1, Functional Grade, clone: PK136, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-5941-85

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15238707

  • POA / 500µg

This item cannot be ordered online because the price is subject to change. Please contact our Product Support team on 01509 555500 for assistance.

Disclaimers:

For Research Use Only.


Description

Description

Description: The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in the NK1.1 mouse strains including C57BL and NZB. Several commonly used laboratory mouse strains such as BALB/c, SJL, AKR, CBA, C3H and A do not express the NK1.1 antigen. For detection of NK cells in these strains the monoclonal antibody DX5 (Cat. No. 14-5971) should be used. Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5+PK136- and DX5-PK136+ cells.Applications Reported: PK136 has been reported for use in flow cytometric analysis, depletion of NK cells, and in vitro functional studies.Applications Tested: The PK136 antibody has been tested by flow cytometric analysis of C57Bl/6 mouse splenocytes and can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in other applications.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

NK1.1
PK136
Unconjugated
Purified
Murine
500μg
Store at 2-8°C.
Monoclonal
Functional Assay
1mg/mL
aqueous buffer, no sodium azide
CD161, NKR-P1C, Ly-55
IgG2a
RUO
Primary
Murine
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