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NK1.1 Monoclonal Antibody (PK136), Alexa Fluor™ 700, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 56-5941-82
114.00 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.
Code : NEW
Additional Details : Weight : 0.09500kg
Description
Description: The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in the NK1.1 mouse strains including C57BL and NZB. Several commonly used laboratory mouse strains such as BALB/c, SJL, AKR, CBA, C3H and A do not express the NK1.1 antigen. For detection of NK cells in these strains the monoclonal antibody DX5 (Cat. No. 14-5971) should be used. Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5+PK136- and DX5-PK136+ cells. Applications Reported: This PK136 antibody has been reported for use in flow cytometric analysis. Applications Tested: This PK136 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Alexa Fluor™ 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.
NK1.1 Monoclonal antibody specifically detects NK1.1 in Mouse samples. It is validated for Flow Cytometry.Specifications
NK1.1 | |
Monoclonal | |
0.2 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
P27812, P27814 | |
Klrb1b, Klrb1c | |
Affinity chromatography | |
RUO | |
17059, 80782 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
PK136 | |
Alexa Fluor 700 | |
Klrb1c | |
AI462337; CD161; CD161 antigen-like family member B; CD161 antigen-like family member C; CD161b; CD161c; EMBL:AAQ11375.1}; Inhibitory receptor NKR-P1B; killer cell lectin-like receptor subfamily A member 1C; killer cell lectin-like receptor subfamily B member 1B; killer cell lectin-like receptor subfamily B member 1B allele A; Killer cell lectin-like receptor subfamily B member 1B allele B; killer cell lectin-like receptor subfamily B member 1B allele C; killer cell lectin-like receptor subfamily B member 1C; killer cell lectin-like receptor subfamily B member 1D; killer cell lectin-like receptor subfamily B member 1F; KLRB1; Klrb1b; klrb1b {ECO:0000312; Klrb1c; Klrb1d; Klrb1f; klrb1f {ECO:0000250; Ly55; Ly-55; Ly55b; ly-55b; Ly55c; ly-55c; Ly55d; Ly-55d; Ly59; Ly-59; lymphocyte antigen 55 complex, locus B; lymphocyte antigen 55 complex, locus C; lymphocyte antigen 55 complex, locus D; lymphocyte antigen 55b; lymphocyte antigen 55c; Lymphocyte antigen 55d; lymphocyte antigen 59; MGC163 | |
Mouse | |
100 μg | |
Primary | |
Mouse | |
Antibody | |
IgG2a κ |
For Research Use Only.