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NK1.1, Alexa Fluor 700, clone: PK136, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 56-5941-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15540367

  • £140.71 / 100µg


EU Stock 1
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Description

Description

Description: The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in the NK1.1 mouse strains including C57BL and NZB. Several commonly used laboratory mouse strains such as BALB/c, SJL, AKR, CBA, C3H and A do not express the NK1.1 antigen. For detection of NK cells in these strains the monoclonal antibody DX5 (Cat. No. 14-5971) should be used. Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5+PK136- and DX5-PK136+ cells.Applications Reported: This PK136 antibody has been reported for use in flow cytometric analysis.Applications Tested: This PK136 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

NK1.1
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P27814
Klrb1d, Ly55b, Ly55d, MGC163757, MGC163759, NKR-P1B, NKR-P1D, Nkrp1-b, Nkrp1d
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
PK136
Alexa Fluor 700
Klrb1c
Conjugated
Klrb1c
IgG2a, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
17059
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