The NheI restriction enzyme recognizes G^CTAGC sites and cuts best at 37°C in Tango buffer (Isoschizomers: AsuNHI, BmtI).

Brand: Thermo Scientific

Manufacturer Part Number: ER0971

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.00900kg

Product Code. 10704111

Quantity Price
1 £ 26.08 / 500 units
EU Stock 32
Add to basket

Description and Specification


Product Name NheI
Concentration 10U/┬ÁL
Units 500U


  • Superior quality – stringent quality control and industry leading manufacturing process
  • Convenient color-coded Five Buffer System
  • Includes universal Tango buffer for double-digestions
  • BSA premixed in reaction buffers
  • Wide selection of restriction endonuclease specificities


  • Molecular cloning
  • Restriction site mapping
  • Genotyping
  • Southern Blot
  • Restriction fragment length polymorphism (RFLP)
  • SNP


Low salt, high glycerol (> 5%) concentrations, pH > 8.0 or a large excess of enzyme may result in star activity. NheI is inhibited by salt concentrations above 100 mM. Supercoiled plasmids may require up to 10-fold more NheI for complete digestion than linear DNAs (e.g. 10 units are required to cleave 1 µg of.)