missing translation for 'onlineSavingsMsg'
Learn More

CD271 (NGF Receptor), clone: ME20.4, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-9400-80

Code : NEW

Additional Details : Weight : 0.09500kg

 View more versions of this product

Product Code. 15537596

  • £63.17 / 25µg


EU Stock 2
Add to basket

Description

Description

Description: The monoclonal antibody ME20.4 recognizes CD271, a 75kDa type I transmembrane protein that belongs to the low-affinity neurotrophin receptor and tumor necrosis factor receptor superfamily. CD271 is expressed by neurons, Schwann cells, melanocytes, mesenchymal stem/stromal cells, neural crest stem cells, astrocytes, oligodendrocytes, and a subset of lymphoid cells including follicular dendritic cells. CD271 binds to the ligands NGF, BDNF, NTF3, and NTF4. CD271 contains a death domain which induces apoptosis and regulates cell growth and proliferation through interactions with TRAF2, TRAF4, TRAF6, PTPN13, and RANBP9.The ME20.4 monoclonal antibody recognizes human, non-human primate, mouse, rat, sheep, rabbit, cat, dog, and pig CD271.Applications Reported: This ME20.4 antibody has been reported for use in flow cytometric analysis, western blotting, microscopy, immunohistochemical staining, and immunocytochemistry.Applications Tested: This ME20.4 antibody has been tested by flow cytometric analysis of SK-N-SH cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This ME20.4 antibody has also been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

NGFR
ME20.4
Unconjugated
Purified
IgG1, kappa
RUO
Primary
Sheep
Western Blot
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Murine
25μg
Store at 2-8°C.
Monoclonal
Videos

For Research Use Only.