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Nestin, eFluor 570, clone: 10C2, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 41-9843-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15508986

  • £89.95 / 25µg


Estimated Shipment date
from Supplier 25-07-2018
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Description

Description

Description: The monoclonal antibody 10C2 recognizes human nestin (residues 1464-1614). Nestin is a 220-240kDa Class VI intermediate filament protein that is expressed in stem cells of the developing nervous system. Expression has also been found in pancreatic islet and mesenchymal precursors in addition to cells with angiogenic potential. Upon differentiation, nestin-containing filaments are replaced by cell type-specific intermediate filament proteins, such as GFAP. Expression has been identified in a variety of cancerous cells including glioblastomas and pancreatic tumors. No cross-reactivity to rodent nestins has been observed using the 10C2 antibody.Applications Reported: This 10C2 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.Applications Tested: This 10C2 antibody has been tested by immunocytochemistry of formaldehyde-fixed and permeabilized human cells and can be used at less than or equal to 1 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.Excitation: 555 nm; Emission: 570 nm
Specifications

Specifications

Nestin
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P48681
FLJ21841, Nbla00170
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
ImmunocytochemistryþImmunohistochemistryþImmunohistochemistry (Frozen)þMicroscopy
10C2
eFluor 570
NES
Conjugated
NES
IgG1
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
10763
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