Nestin, Alexa Fluor 488, clone: 10C2, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 53-9843-82
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: The monoclonal antibody 10C2 recognizes human nestin (residues 1464-1614). Nestin is a 220-240kDa Class VI intermediate filament protein that is expressed in stem cells of the developing nervous system. Expression has also been found in pancreatic islet and mesenchymal precursors in addition to cells with angiogenic potential. Upon differentiation, nestin-containing filaments are replaced by cell type-specific intermediate filament proteins, such as GFAP. Expression has been identified in a variety of cancerous cells including glioblastomas and pancreatic tumors. No cross-reactivity to rodent nestins has been observed using the 10C2 antibody.Applications Reported: This 10C2 antibody has been reported for use in microscopy, immunocytochemistry, immunohistochemical staining, and flow cytometric analysis.Applications Tested: This 10C2 antibody has been tested by immunocytochemistry of formaldehyde-fixed and permeabilized cells. This can be used at less than or equal to 10 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|Alexa Fluor 488|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.