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Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Kit

Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit.

Brand:  Thermo Scientific™ 78833

Code : PN

Additional Details : CAS Number : 813-19-4 Weight : 0.80000kg


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Product Code. 11865694

  • £226.03 / 15mL


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Description

Description

The NE-PER Kit is a nuclear protein extraction method that involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilize and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. Once desalted or diluted, the isolated proteins can be used to perform immunoassays and protein interaction experiments, such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.

Highlights:

  • Fast – obtain nuclear and cytoplasmic fractions in less than two hours

  • Proven – the NE-PER Kit is referenced in more than 950 distinct publications

  • Scalable – two kit sizes for producing extracts from cells and tissues

  • Convenient – simple instructions do not require ultracentrifugation over gradients

  • Compatible – use for downstream assays, including Western blotting, gel-shift assays, protein assays, reporter gene assays and enzyme activity assays

Includes:

Two kit sizes, each containing three buffer and detergent solutions

Compatible with:

Thermo Scientific LightShift Chemiluminescent EMSA Kit (Mfr. No. 20148)

Specifications

Specifications

Three-component kit
15mL kit
Cytoplasmic Extraction Reagent I (CER I), 10mL, Cytoplasmic Extraction Reagent II (CER II), 0.55mL, Nuclear Extraction Reagent (NER), 5mL
Nuclear
NE-PER Nuclear and Cytoplasmic Extraction Kit
Cultured mammalian cells
50 samples each containing 2 million cells (20uL packed)
Videos
Documentation

Technical Documents

Special Offers

Special Offers

  • Big discounts are available on a range of popular products including chemicals, lab essentials, equipment, consumables, life science and safety products.
    : 13107

    2018-05-31

    *Discount will apply to qualifying orders received by Fisher Scientific between 03/04/2018 and 31/05/2018. Promotion code must be presented at the time of order. Discount applies to the list price in effect at the time the order is received. Promotion cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.

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