Nanog, clone: hNanog.2, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-5768-82
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The hNanog.2 antibody recognizes human Nanog, a homeobox transcription factor expressed in embryonic stem cells (ESC). Nanog is essential for self-renewal of ESC and the inner cell mass and is throught to function in the same capacity as other factors such as Oct4 and Sox2. Nanog plays a crucial role in embryonic cell fate specification following formation of the blastocyst by regulating proliferation and preventing differentiation. Nanog functions by directly binding DNA consensus sequences to activate or repress transcription. In differentiating ESC, Nanog binds to its own promoter leading to repression of expression. Nanog has been reported to be overexpressed in germ cell tumors of the gonads and central nervous system as well as breast and colorectal cancer.Applications Reported: This hNanog.2 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.Applications Tested: This hNanog.2 antibody has been tested by immunoblotting of NTERA cell lysates and can be used at less than or equal to 1 ug/mL. The hNanog.2 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using high or low pH antigen retrieval and can be used at less than or equal to 20 ug/mL. The hNanog.2 antibody has been tested by immunocytochemistry on formaldehyde fixed and permeabilized cells and can be used at less than or equal to 5 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
|Store at 2-8°C.|