eBioscience™ SAFE™ Human VEGF, Recombinant Protein

Human Recombinant Protein

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 68-8784-63

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Product Code. 15550627

Quantity Price
1 £ 97.22 / 10µg
Estimated Shipment date
from Supplier 01-06-2017
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Description and Specification

Specification

Purity ≥95%
Cross Reactivity Human
Recombinant Yes
For Use With (Application) Functional Assays
Biological Activity The ED50 of this protein, as measured by cell proliferation of HUVECS (human umbilical vein endothelial cells), is <10ng/mL. This corresponds to a specific activity of greater than or equal to 1 x 105 Units/mg.
Endotoxin Concentration Less than 0.005ng/μg (0.05 EU/μg) as measured by turbitidity assay
Conjugate Unlabeled
Product Type Recombinant Protein
Source Plant
Molecular Weight 21.3kDa (with glycosylation the apparent molecular weight is 30kDa)
Regulatory Status RUO
Storage Requirements The lyophilized powder can be stored at less than or equal to -20°C. (Short term storage at 2-8°C). Reconstitute in sterile reagent grade deionized water to a concentration no less than 100μg/mL. Once reconstituted, protein should be aliquoted and stored at -20°C or lower. For best stability add 0.1% BSA before freezing aliquots.
Formulation Sterile lyophilized (PBS buffer pH 7.2)
Purification Method SDS-PAGE
Name VEGF
Quantity 10μg
Structural Form Plant expressed 165 amino acids (Ala27-Arg191) with 16 amino acid Histidine tag. Accession NP_001165097

This SAFE (Serum and Animal Free, low Endotoxin) protein has been made in plants with no exposure to animal protein during expression or purification. This animal free product has very low levels of endotoxin.

Vascular endothelial growth factor (VEGF), also called vascular permeability factor (VPF), is produced by cultured vascular smooth muscle cells. From analysis of transcripts from these cells by PCR and cDNA cloning, 3 different forms of the VEGF coding region have been identified. These cDNAs had predicted products of 189, 165, and 121 amino acids. VEGF is a mitogen primarily for vascular endothelial cells. It is structurally related to platelet-derived growth factor. VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. The VEGF receptor, Flk1, is exclusively expressed in endothelial cells. VEGF may be a major regulator of tumor angiogenesis in vivo. VEGF165 is the most common form in most tissues.