eBioscience™ Human LIF, Recombinant Protein

Human Recombinant Protein

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 14-8460-80

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Product Code. 15547536

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Description and Specification

Specification

Regulatory Status RUO
For Use With (Application) Cytokine Bioassays , Functional Assays
Molecular Weight 6.4kDa
Cross Reactivity Human
Structural Form E. coli expressed amino acids Ser23-Phe202, accession number NM_002309
Product Type Recombinant Protein
Biological Activity The ED50 of this protein, as measured by mouse IL-6 induction in M1 cells, is less than or equal to 15ng/mL. This corresponds to a specific activity of greater than or equal to 6.7 x 104 Units/mg.
Purification Method SDS-PAGE
Storage Requirements Store at less than or equal to -70°C.
Conjugate Unlabeled
Source E. coli
Accession Number NM_002309
Quantity 25μg
Endotoxin Concentration Less than 0.01ng/μg cytokine, as determined by the LAL assay.
Concentration 0.1mg/mL
Gene Symbol Leukemia Inhibitory Factor, HILDA
Name LIF
Recombinant Yes
Purity >97%
Formulation Sterile liquid: 0.1M glycine, 1% BSA, pH 3.0

Leukemia inhibitory factor (LIF) is a 20 kDa protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice, and contribute to stem cell self-renewal. LIF can be up-regulated by pro-inflammatory cytokines such as TNF alpha and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific.