eBioscience™ Mouse TNF alpha, Recombinant Protein

Mouse Recombinant Protein

Brand: eBioscience™

Manufacturer Part Number: 14-8321-63

X10 ug Mouse TNF alpha Recombinant Protein

UNSPSC: 12352200

Code: NEW

Additional Details:
Additional Details: Weight: 0.10000kg

Disclaimers: For Research Use Only.

Product Code. 15255323

Quantity Price
1 £176.10 / Each
Estimated Shipment date
from Supplier 27-10-2016
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Description and Specification


Accession Number NM_013693
Conjugate Unlabeled
Cross Reactivity Mouse
For Use With (Application) Cytokine Bioassays
Formulation Sterile liquid; 10mM sodium phosphate, pH 7.2, 150mM NaCl, 0.5% BSA. 0.22μm filtered.
Molecular Weight The protein is not methionylated at the N-terminal and has a predicted molecular mass of 17,258. The DTT-reduced and non-reduced protein migrate as 17kDa polypeptides on SDS-PAGE.
Name TNF alpha
Purification Method SDS-PAGE
Purity >98%
Quantity 10μg
Recombinant Yes
Source E. coli
Regulatory Status RUO
Structural Form E. coli expressed amino acids Leu 80-Leu 235 of mouse TNF- alpha accession # NM_013693
Endotoxin Concentration Less than 0.01ng/μg cytokine as determined by the LAL assay.
Gene Symbol Tumor Necrosis Factor alpha, TNFa TNF-a
Validated Application FA
Biological Activity The ED50 of this protein, as measured by L929 cytotoxicity assay, is less than or equal to 25pg/mL. This corresponds to a specific activity of greater than or equal to 4 x 107 Units/mg.

Mouse TNF-a is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 26 kDa form of TNF-α is expressed as a membrane-bound molecule. TNF-α is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF-α have been observed.