eBioscience™ Mouse TNF alpha, Recombinant Protein

Mouse Recombinant Protein

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 14-8321-63

UNSPSC: 12352200

Code: NEW

Additional Details:
Additional Details: Weight: 0.10000kg



Disclaimers: For Research Use Only.

Product Code. 15255323

Quantity Price
1 £ 115.56 / Each
EU Stock 4
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Description and Specification

Specification

For Use With (Application) Cytokine Bioassays
Molecular Weight The protein is not methionylated at the N-terminal and has a predicted molecular mass of 17,258. The DTT-reduced and non-reduced protein migrate as 17kDa polypeptides on SDS-PAGE.
Regulatory Status RUO
Name TNF alpha
Biological Activity The ED50 of this protein, as measured by L929 cytotoxicity assay, is less than or equal to 25pg/mL. This corresponds to a specific activity of greater than or equal to 4 x 107 Units/mg.
Endotoxin Concentration Less than 0.01ng/μg cytokine as determined by the LAL assay.
Validated Application FA
Gene Symbol Tumor Necrosis Factor alpha, TNFa TNF-a
Source E. coli
Structural Form E. coli expressed amino acids Leu 80-Leu 235 of mouse TNF- alpha accession # NM_013693
Accession Number NM_013693
Conjugate Unlabeled
Cross Reactivity Mouse
Formulation Sterile liquid; 10mM sodium phosphate, pH 7.2, 150mM NaCl, 0.5% BSA. 0.22μm filtered.
Recombinant Yes
Quantity 10μg
Purity >98%
Purification Method SDS-PAGE

Mouse TNF-a is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 26 kDa form of TNF-α is expressed as a membrane-bound molecule. TNF-α is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF-α have been observed.