Invitrogen™ Mouse TNF alpha, Recombinant Protein

Recombinant Protein

Product Code	Brand	Quantity
15587516	Affymetrix eBioscience 14-8321-62	5μg
15255323	Affymetrix eBioscience 14-8321-63	10μg

Description

Tumor Necrosis Factor-Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor-bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single ∽17kDa band following SDS-PAGE under non-reducing conditions. Description: Mouse TNF-a is a 17kDa factor produced by macrophages, monocytes, neutrophils, CD4^+ T cells and NK cells. A 26kDa form of TNF-alpha is expressed as a membrane-bound molecule. TNF-alpha is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF-alpha have been observed. Applications Reported: Recombinant mouse TNF alpha is biologically active and can induce cytolysis of the L929 mouse cell line in culture. Applications Tested: The ED50 of this protein, as measured by L929 cytotoxicity assay, is less than or equal to 25 pg/mL. This corresponds to a specific activity of greater than or equal to 4 x 10e7 Units/mg. Source: E. coli expressed amino acids Leu 80-Leu 235 of mouse TNF-alpha accession # NM_013693. Bioactivity: The ED50 of this protein, as measured by L929 cytotoxicity assay, is less than or equal to 25 pg/mL. This corresponds to a specific activity of greater than or equal to 4 x 10e7 Units/mg. Endotoxin: Less than 0.01ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE. Molecular Weight: The protein is not methionylated at the N-terminal and has a predicted molecular mass of 17,258. The DTT-reduced and non-reduced protein migrate as 17kDa polypeptides on SDS-PAGE. Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Molecular Weight (g/mol): The protein is not methionylated at the N-terminal and has a predicted molecular mass of 17,258. The DTT-reduced and non-reduced protein migrate as 17kDa polypeptides on SDS-PAGE.
• Source: E. coli
• Structural Form: E. coli expressed amino acids Leu 80-Leu 235 of mouse TNF- alpha accession # NM_013693
• Biological Activity: The ED50 of this protein, as measured by L929 cytotoxicity assay, is less than or equal to 25pg/mL. This corresponds to a specific activity of greater than or equal to 4 x 10⁷ Units/mg.
• Recombinant: Recombinant

• Purification Method: SDS-PAGE
• Regulatory Status: RUO
• Endotoxin Concentration: Less than 0.01ng/μg cytokine as determined by the LAL assay.
• Conjugate: Unconjugated

For Research Use Only.