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Invitrogen™ TNF alpha Mouse ELISA Kit

For the quantitative detection of mouse TNF-alpha.

£451.00 - £2400.00

Specifications

Accession Number P06804
Assay Range 31.3-2,000 pg/mL
Assay Sensitivity 3.7 pg/mL
Conjugate Biotin
Product Type ELISA Kit
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Includes: Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to mouse TNF-alpha
Biotin-Conjugate anti-mouse TNF-alpha polyclonal antibody
Streptavidin-HRP
Mouse TNF-alpha Standard lyophilized, 2ng/mL upon reconstitution
Calibrator Diluent
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

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Product Code Brand Quantity Price Quantity & Availability  
15562017
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Invitrogen™
BMS607-3
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Estimated Shipment: 08-04-2024
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Invitrogen™
BMS607-3TWO
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£580.00
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BMS607-3FIVE
5 x 96 Tests
£1444.00
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Invitrogen™
BMS607-3TEN
10 x 96 Tests
£2400.00
Pack of 10
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Estimated Shipment: 22-04-2024
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Description

Description

The Mouse Tumor Necrosis Factor alpha (Ms TNFα) ELISA quantitates Ms TNFα in mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms TNFα. Principle of the method The Mouse TNFα solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Platinum Kits undergo stringent in-house development, manufacture and quality-validation processes ensuring highest possible product performance and optimal lot-to-lot consistency
Specifications

Specifications

P06804
3.7 pg/mL
ELISA Kit
Mouse
Colorimetric Microplate Reader
DIF,TNF-a,TNF-alpha,TNFSF2,TNFalphaa,Tnfsf1a,Tnlg1f,Tnf
6.5%
HRP
Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
Cachectin, Tumor necrosis factor alpha, DIF
1 hr. 20 min.
31.3-2,000 pg/mL
Biotin
Plasma, Serum, Supernatant
ELISA
21926
5.7%
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
RUO
2°C to 8°C
Mouse
3 hr. 30 min.
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