Invitrogen™ IL-17 Mouse ELISA Kit

For the quantitative detection of mouse TNF-alpha.

Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse TNF-alpha
Biotin-Conjugate anti-mouse TNF-alpha monoclonal antibody
Streptavidin-HRP
Mouse TNF-alpha Standard lyophilized, 200pg/mL upon reconstitution
Calibrator Diluent
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Amplification Diluent Concentrate (2x)
Amplification Reagent I
Amplification Reagent II
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films

Product Code	Brand	Quantity
15551957	Invitrogen™ BMS6001	96 Tests
15561957	Invitrogen™ BMS6001TEN	10 x 96 Tests

Description

The Mouse Interleukin-17 (Ms IL-17) ELISA quantitates Ms IL-17 in mouse serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-17. Principle of the method The Mouse IL-17 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Mouse IL-17 mRNA is specifically expressed by activated alpha beta TCR + CD2-CD8-T-cells, a small subset with a potentially important role in immune regulation. IL-17 induces the secretion of IL-6, IL-8, PGE2, MCP-1 and G-CSF by adherent cells like fibroblasts, keratinocytes, epithelial and endothelial cells. IL-17 is also able to induce ICAM-1 surface expression of T-cells. IL-17 has been shown to support the growth of hemopoietic progenitors, it has stimulatory effects on granulopoiesis in mice.

IL-17 may affect osteoclastic resorption and thereby mediate bone destruction accompanying some inflammatory diseases. Astrocytes produce NO when stimulated by IL-17, which suggests a possible role for IL-17 in the inflammatory diseases of the CNS. Forced expression of murine IL-17 induces immune responses and multiorgan inflammation.

Interleukin-17 is involved in the regulation of hematopoiesis and its effects are dependent on the physiological/pathological status of the organism. IL-17 expression in airways is upregulated upon allergen exposure like in allergic asthma. Its crucial role in T-cell activation suggests that IL-17 is involved in the development of autoimmune arthritis.

Specifications

• Accession Number: Q62386
• Assay Sensitivity: 1.6 pg/mL
• Product Type: ELISA Kit
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: Ctla-8,Ctla8,IL-17,IL-17A,Il17
• Intraassay CV: 9.1%
• Label or Dye: HRP
• Sample Volume: Serum, 50 μL; Supernatant, 50 μL
• Synonym: Interleukin-17, IL-17A, CTLA8
• Test Time: 1 hr. 20 min.

• Assay Range: 7.8-500 pg/mL
• Conjugate: Biotin
• Sample Type: Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 16171
• Interassay CV: 8.5%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Mouse
• Total Assay Time: 3 hr. 10 min.

For Research Use Only