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Thermo Scientific™ Pierce™ Mouse TNF alpha ELISA Kits

Measure levels of mouse tumor necrosis factor alpha (TNFa) in mouse serum and cell culture supernatant samples using 96-well plates.

Brand:  Thermo Scientific™ Pierce™ EMTNFA

Code : PN

Additional Details : Weight : 0.45000kg Transport : UN number : 2811 Chem class : 6.1 Pack group : III

Product Code. 10475725

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance


Description

Description

These mouse TNF alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The antibody-pair kit consists of the matched TNFα antibody pairs and TNFα standard only. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant mouse TNFα (mouse tumor necrosis factor alpha)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96 well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 50 to 2450pg/mL

  • Sensitivity: < 9pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with mouse IL-2, IL-3, IL-4, IL-5, IL-6, GM-CSF, IFNα or human TNFα. This ELISA has some cross-reactivity with rabbit and cynomologous monkey TNFα in culture supernatants

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the antibody-pair kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).

Specifications

Specifications

50 to 2450pg/mL
Colorimetric ELISA (TMB substrate) in clear 96 well strip plate
Lyophilized Recombinant Mouse TNF alpha Standard, 2 vials, TMB Substrate Solution, 13mL, Streptavidin-HRP Concentrate, 100µL, Standard Diluent, 12mL, HRP Dilution Buffer, 14mL, Biotinylated Antibody Reagent, 8mL, Anti-Mouse TNF alpha Precoated 96-well Strip Plate, 1 ea, Adhesive Plate Covers, 6 ea, 30X Wash Buffer, 50mL, Stop Solution, 13mL
Absorbance at 450nm minus absorbance at 550nm
<9pg/mL
50µL
96 microplate wells
Natural and recombinant mouse TNFa (mouse tumor necrosis factor alpha)
Mouse TNF alpha ELISA Kit
Complete ELISA kit with coated 96-well plate
Intra-assay CV <10%; inter-assay CV <10%
1 plate kit
Cell culture supernatant, serum
No significant cross-reaction with mouse IL-2, IL-3, IL-4, IL-5, IL-6, GM-CSF, IFNa or human TNFa. This ELISA has some cross-reactivity with rabbit and cynomologous monkey TNFa in culture supernatants
3 hr.
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