Thermo Scientific™ Pierce™ Mouse TNF alpha ELISA Kits

Measure levels of mouse tumor necrosis factor alpha (TNFa) in mouse serum and cell culture supernatant samples using 96-well plates.

Overview
Brand: Thermo Scientific™ Pierce™

Manufacturer Part Number: EMTNFA

UNSPSC: 41116126

Code: PN

Additional Details:
Additional Details: Weight: 0.45000kg Transport: UN number : 2811 Chem class : 1 Pack group : III



Product Code. 10475725

Quantity Price
1 £ 416.0 / 1 set
Estimated Shipment date
from Supplier 03-04-2017
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Description and Specification

Specification

Sensitivity <9pg/mL
Precision Intra-assay CV <10%; inter-assay CV <10%
Measurement Absorbance at 450nm minus absorbance at 550nm
Formulation Complete ELISA kit with coated 96-well plate
Kit Contents 30X Wash Buffer, 50mL
Assay Range 50 to 2450pg/mL
Sufficient For 96 microplate wells
Format Colorimetric ELISA (TMB substrate) in clear 96 well strip plate
Specificity No significant cross-reaction with mouse IL-2, IL-3, IL-4, IL-5, IL-6, GM-CSF, IFNa or human TNFa. This ELISA has some cross-reactivity with rabbit and cynomologous monkey TNFa in culture supernatants
Target Natural and recombinant mouse TNFa (mouse tumor necrosis factor alpha)
Sample Size 50µL
Description Mouse TNF alpha ELISA Kit
Sample Type Cell culture supernatant, serum
Total Assay Time 3 hr.
Quantity 1 plate kit

These mouse TNF alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The antibody-pair kit consists of the matched TNFα antibody pairs and TNFα standard only. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant mouse TNFα (mouse tumor necrosis factor alpha)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96 well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 50 to 2450pg/mL

  • Sensitivity: < 9pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with mouse IL-2, IL-3, IL-4, IL-5, IL-6, GM-CSF, IFNα or human TNFα. This ELISA has some cross-reactivity with rabbit and cynomologous monkey TNFα in culture supernatants

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the antibody-pair kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).

Documentation

Technical documents