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Invitrogen™ TGF beta-1 Mouse ELISA Kit

For the quantitative detection of mouse TGF-beta1.

Brand:  Invitrogen™ BMS608-4FIVE

962.71 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15522027

  • £1444.00 / Pack of 5
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Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse TGF-beta1
Biotin-Conjugate anti-mouse TGF-beta1 polyclonal antibody
Streptavidin-HRP
Mouse TGF-beta1 Standard lyophilized, 4ng/mL upon reconstitution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
]Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films

Description

Description

The Mouse Transforming Growth Factor beta 1 (Ms TGFβ1) ELISA quantitates Ms TGFβ1in Mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Mouse TGFβ1. Principle of the method The Mouse TGFβ1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

TGF beta-1 (TGFB1, Transforming Growth Factor Beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its pro-domain. Activation of TGF beta-1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF beta-1, an amino-terminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP. TGF beta-1 helps regulates proliferation, differentiation, adhesion, migration in many cell types. Many cells have TGF beta receptors, and the protein positively and negatively regulates many other growth factors. TGF beta-1 is cleaved into a latency-associated peptide and a mature TGF beta-1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGF beta-1 homodimer. The mature peptide may also form heterodimers with other TGF beta family members. The gene for TGF beta-1 is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease and cystic fibrosis.
Specifications

Specifications

P04202
7.8 pg/mL
ELISA Kit
Mouse
Colorimetric Microplate Reader
TGF-beta1,TGFbeta1,Tgfb,Tgfb-1
7.9%
HRP
RUO
2°C to 8°C
Mouse
4 hr.
31.25-2,000 pg/mL
Biotin
Plasma, Serum, Supernatant
ELISA
21803
5.8%
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
5 x 96 Tests
Plasma, 20 μL; Serum, 20 μL; Supernatant, 20 μL
Camurati-Engelmann disease, DPD1
1 hr. 20 min.
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