Invitrogen™ eBioscience™ Mouse TGF beta 1, Recombinant Protein, Carrier-Free
Mouse Recombinant Protein
Brand: Invitrogen™ 34-8342-82
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionMouse TGF beta 1 (Transforming Growth Factor beta 1) is a member of a superfamily of homologous, disulfide-linked, homodimeric proteins that regulate the proliferation and differentiation of normal and transformed cells. Human TGF beta 1 is a 25 kDa protein with each subunit containing 112 aa residues. TGF beta 1 is secreted from the many cell types in a complex with LAP (Latency Associated Peptide) and also with LTBP (latent TGF beta binding protein). Disassociation is thought to happen in the extracellular matrix via proteases and/or integrins. Regulatory T cells are a recently identified source of TGF beta 1 thought to be a key player in their suppressive functions. TGF beta 1 binds through TGF beta receptors (of which there is a large family). The protein is highly conserved across all mammalian species.
|Functional Assays, Cell Culture|
|Mature TGFb1 polypeptide has a predicted molecular mass of 12,794. On reducing SDS-PAGE the protein migrates as a 13kDa polypeptide and on non-reducing SDS page the cystine-linked homodimer migrates as a 25kDa protein.|
|Less than 0.01ng/μg cytokine as determined by the LAL assay.|
|Sterile liquid; 0.1M Gly-HCl, pH 3.0. 0.22 µm
|TGF beta 1|
|TGFb1 was expressed in CHO cells.
Mature TGFb1 (ala279 - ser390; accession #BC001180) was
purified from the conditioned media.
|Transforming growth factor beta-1|
|The ED50 of this protein, as measured by inhibition of IL-4 induced proliferation in HT-2 cells, is less than or equal to 40pg/mL. This corresponds to a specific activity of greater than or equal to 2.5 x 107 units/mg.|