eBioscience™ Mouse TGF beta 1, Recombinant Protein, Carrier-Free

Mouse Recombinant Protein

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 34-8342-82

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Disclaimers: For Research Use Only.

Product Code. 15518796

Quantity Price
1 £ 769.77 / 100µg
EU Stock 6
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Description and Specification

Specification

Concentration 0.5mg/mL
Structural Form TGFb1 was expressed in CHO cells.
Mature TGFb1 (ala279 - ser390; accession #BC001180) was
purified from the conditioned media.
Purity >95%
Biological Activity The ED50 of this protein, as measured by inhibition of IL-4 induced proliferation in HT-2 cells, is less than or equal to 40pg/mL. This corresponds to a specific activity of greater than or equal to 2.5 x 107 units/mg.
Formulation Sterile liquid; 0.1M Gly-HCl, pH 3.0. 0.22 µm
filtered.
Conjugate Unlabeled
Endotoxin Concentration Less than 0.01ng/μg cytokine as determined by the LAL assay.
Quantity 100μg
Regulatory Status RUO
Molecular Weight Mature TGFb1 polypeptide has a predicted molecular mass of 12,794. On reducing SDS-PAGE the protein migrates as a 13kDa polypeptide and on non-reducing SDS page the cystine-linked homodimer migrates as a 25kDa protein.
Recombinant Yes
Name TGF beta 1
Purification Method SDS-PAGE
Validated Application FA
Gene Symbol Transforming growth factor beta-1
For Use With (Application) Functional Assays, Cell Culture
Accession Number BC001180
Cross Reactivity Mouse
Source CHO Cells

Mouse TGF beta 1 (Transforming Growth Factor beta 1) is a member of a superfamily of homologous, disulfide-linked, homodimeric proteins that regulate the proliferation and differentiation of normal and transformed cells. Human TGF beta 1 is a 25 kDa protein with each subunit containing 112 aa residues. TGF beta 1 is secreted from the many cell types in a complex with LAP (Latency Associated Peptide) and also with LTBP (latent TGF beta binding protein). Disassociation is thought to happen in the extracellular matrix via proteases and/or integrins. Regulatory T cells are a recently identified source of TGF beta 1 thought to be a key player in their suppressive functions. TGF beta 1 binds through TGF beta receptors (of which there is a large family). The protein is highly conserved across all mammalian species.