Invitrogen™ IP-10 (CXCL10) Mouse ELISA Kit

For the quantitative detection of mouse IP-10.

Brand:  Invitrogen™ BMS6018

Product Code. 15581977

Includes: Aluminum pouch(es) with a Microwell Plate coated with polyclonal antibody to mouse IP-10
Biotin-Conjugate anti-mouse IP-10 polyclonal antibody
Streptavidin-HRP
Mouse IP-10 Standard lyophilized, 1000pg/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Description

The Mouse Inflammatory Protein 10/CXCL10 (Ms IP-10) ELISA quantitates Ms IP-10 in mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IP-10. Principle of the method The Mouse IP-10 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CXCL10 ( C-X-C Motif Chemokine Ligand 10) is a 10 kDa protein and member of the CXC family that acts by binding to CXCR3. Binding of CXCL10 to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. CXCL10 is a pro-inflammatory chemoattractant cytokine that attracts activated T lymphocytes. CXCL10 is constitutively expressed at low levels in lymph organs, and the expression is highly induced by IFN- gamma and to some extent by IL12. It is an important biomarker for diagnosis of many clinical conditions, and it plays an important role in host defense and inflammation. CXCL10 exhibits an angiostatic and an antitumor role that is also involved in Th1 inflammatory diseases, Hashimoto's thyroiditis, Graves′ disease and Type 1 diabetes mellitus.

Specifications

• Accession Number: P17515
• Assay Sensitivity: 6.5 pg/mL
• Product Type: ELISA Kit
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: C7,CRG-2,INP10,IP-10,IP10,Ifi10,Scyb10,gIP-10,mob-1
• Intraassay CV: 8.4%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Mouse
• Total Assay Time: 3 hr. 10 min.

• Assay Range: 7.8-500 pg/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 15945
• Interassay CV: 7.2%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
• Synonym: Interferon-gamma-inducible protein 10, CXCL10
• Test Time: 1 hr. 20 min.

For Research Use Only