missing translation for 'onlineSavingsMsg'
Learn More

Mouse HMGB1 Carrier-Free (High Mobility Group Box 1), Recombinant Protein, eBioscience™

Recombinant Protein

Brand:  Affymetrix eBioscience 34-8401-82

Code : NEW

Additional Details : Weight : 0.09500kg

 View more versions of this product

Product Code. 15598796

  • £797.72 / 100µg


Estimated Shipment date
from Supplier 05-07-2018
Add to basket

Description

Description

High-mobility group box-1 (HMGB1) protein was originally described as a nuclear non-histone DNA binding chromosomal protein. However, recent studies indicate that damaged, necrotic cells liberate HMGB1 into the extracellular milieu where it functions as a proinflammatory cytokine. Mouse HMGB1 is expressed as a 215 amino acid single chain polypeptide containing three domains: two tandem-linked positively charged DNA-binding domains (HMG box A, aa 9-79; and box B, aa 89-162), and a negatively charged 30 aa C-terminal acidic tail region. Residues 28 - 44 and 180 - 185 contain a nuclear localization signal (NLS). The cytokine activity of HMGB1 is contained in the B box, while the A box is associated with the helix-loop-helix domain of transcription factors. HMGB1 acts both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, as well as a mediator of T cell-dendritic cell interaction. HMGB1 is secreted and acts to transduce cellular signals through its high affinity receptor, RAGE and possibly, TLR2 and TLR4. Description: High-mobility group box-1 (HMGB1) protein was originally described as a nuclear non-histone DNA binding chromosomal protein. However, recent studies indicate that damaged, necrotic cells liberate HMGB1 into the extracellular milieu where it functions as a proinflammatory cytokine. Mouse HMGB1 is expressed as a 215 amino acid single chain polypeptide containing three domains: two tandem-linked positively charged DNA-binding domains (HMG box A, aa 9-79; and box B, aa 89-162), and a negatively charged 30 aa C-terminal acidic tail region. Residues 28 - 44 and 180 - 185 contain a nuclear localization signal (NLS). The cytokine activity of HMGB1 is contained in the B box, while the A box is associated with the helix-loop-helix domain of transcription factors. HMGB1 acts both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, as well as a mediator of T cell-dendritic cell interaction. HMGB1 is secreted and acts to transduce cellular signals through its high affinity receptor, RAGE and possibly, TLR2 and TLR4. Applications Reported: Mouse HMGB1 Recombinant Protein Carrier-Free is biologically active. Applications Tested: The ED50 of this protein, as measured by mouse TNF alpha induction in RAW264.7 cells, is 3-25μg/mL in the presence of 10μg/mL Polymyxin B. This corresponds to a specific activity of 3,300-40 Units/mg. Source: E. coli expressed amino acids Met1-Glu215 of mouse HMGB-1 accession # NM_010439. Bioactivity: The ED50 of this protein, as measured by mouse TNF alpha induction in RAW264.7 cells, is 3-25 ug/mL in the presence of 10 ug/mL Polymyxin B. This corresponds to a specific activity of 3,300-40 Units/mg. Endotoxin: Less than 0.01ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE. Molecular Weight: The protein contains an N-terminal His tag and has a predicted molecular mass of 27.6 kDa. Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.
Specifications

Specifications

P63158
Cytokine Bioassays
20mM tris HCl with 0.2M NaCl, 1mM DTT and no preservative; pH 8.0
The protein contains an N-terminal His tag and has a predicted molecular mass of 27.6kDa.
8.0
Affinity chromatography
E. coli
RUO
Recombinant
Mouse HMGB1 Carrier-Free (High Mobility Group Box 1)
Control, Functional Assay
Recombinant Protein, Carrier-Free
Unlabeled
E. coli
0.5mg/mL
Liquid
15289
Mouse HMGB1 Carrier-Free (High Mobility Group Box 1)
SDS-PAGE
100μg
-80°C
E. coli expressed amino acids Met1-Glu215 of mouse HMGB-1 accession # NM_010439
Less than 0.01ng/μg cytokine as determined by the LAL assay.
Hmgb1
The ED50 of this protein, as measured by mouse TNF alpha induction in RAW264.7 cells, is 3-25μg/mL in the presence of 10μg/mL Polymyxin B. This corresponds to a specific activity of 3,300-40 Units/mg.
Mouse
Ms
Recombinant
Videos