Invitrogen™ IL-6 Mouse ELISA Kit

Specifically engineered for accurate and precise measurement of IL-6 protein levels from samples including serum, plasma, and supernatants from cell cultures

Brand:  Invitrogen™ BMS603-2

Product Code. 15522007

Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IL-6
Biotin-Conjugate anti-mouse IL-6 monoclonal antibody
Streptavidin-HRP
Mouse IL-6 Standard lyophilized, 4ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Description

Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury,and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts,activated T cells,activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells,T cells,B cells,and hepatocytes. In addition,IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation,IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor,alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.

Specifications

• Accession Number: P08505
• Assay Sensitivity: 6.5pg/mL
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 16193
• Interassay CV: 8.9%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Mouse
• Total Assay Time: 3 hr. 30 min.

• Assay Range: 31.3-2,000pg/mL
• Product Type: ELISA Kit
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: Il6
• Intraassay CV: 5%
• Quantity: 96 Tests
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
• Synonym: Interferon-beta 2, B-cell stimulatory factor 2,HGF
• Test Time: 1 hr. 20 min.

For Research Use Only