Invitrogen™ Mouse IL-6 (ELISA Standard) Recombinant Protein, eBioscience™

Recombinant Protein

Brand:  Invitrogen™ 29-8061-65

Product Code. 15578716

Description

Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis. Description: Mouse IL-6 (also known as B Cell Stimulating Factor, BCSF, and BSF-2) is a ∽21-28 kDa factor produced by a variety of cell types, including monocytes, fibroblasts and endothelial cells. Upon stimulation, macrophages, T, B, mast, and glial cells, eosinophils, keratinocytes and granulocytes also secrete IL-6. This pleiotrophic cytokine influences the antigen-specific immune response, inflammatory response, and the development of neurons, and is a major mediator of the acute phase reaction. Applications Reported: Mouse IL-6 Lyophilized Standard has been reported for use in the Mouse IL-6 ELISA Ready-SET-Go! Set (cat. 88-7064). Applications Tested: The intended use of the Mouse IL-6 Lyophilized Standard is as a standard for Mouse IL-6 ELISA (cat. 88-7064). Typically the standard is run in a series of 7 doubling dilutions from the ′top′ standard. Please refer to the Certificate of Analysis included with each ELISA set for instructions on the proper reconstitution and dilution of the lyophilized standard. Note: These are single-use vials. Use one time within 4 hours of reconstitution and discard. Reconstitute and dilute as suggested in the Certificate of Analysis or recovery may not be accurate. Source: E. coli expressed amino acids Phe 25-Thre 211 of mature mouse IL-6 accession &Num; MMINTL6.

Specifications

• Accession Number: P08505
• Formulation: protein with no preservative
• Name: Mouse IL-6 ELISA Standard
• Source: E. Coli
• Regulatory Status: RUO
• Gene Symbol: Il6
• Conjugate: Unconjugated
• Species: E. coli
• Form: Lyophilized
• Purity or Quality Grade: Affinity chromatography

• For Use With (Application): Control, ELISA
• Gene ID (Entrez): 16193
• Quantity: 4pack
• Storage Requirements: 4°C
• Common Name: Mouse IL-6 ELISA Standard
• Specific Reactivity: Mouse
• Cross Reactivity: Ms
• Recombinant: Recombinant
• Protein Subtype: Recombinant