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Invitrogen™ Mouse IL-6 ELISA Kits

Measure levels of mouse interleukin-6 in mouse serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples using 96-well plates.

Brand:  Invitrogen™ EM2IL6

Code : PN

Additional Details : Weight : 0.10000kg

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Product Code. 10751175

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Description

Description

These mouse IL-6 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-6 (IL-6) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. The antibody-pair kit consists of the matched IL-6 antibody pairs and IL-6 standard only. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant mouse interleukin-6 (IL-6)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 51 to 2000pg/mL

  • Sensitivity: < 7pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 4 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with mouse GM-CSF, IFNγ, IL-1α, IL-2, IL-3, IL-4, IL-5, IL-7, IL-9, IL-10, IL-12, or human IL-6. This kit has been shown to have a high level of cross-reactivity with recombinant rat IL-6

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the antibody-pair kit.

Includes:The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates.

Specifications

Specifications

51 to 2000pg/mL
Mouse IL-6 ELISA Kit
Complete ELISA kit with coated 96-well plate
Absorbance at 450nm minus absorbance at 550nm
<7pg/mL
50┬ÁL
96 microplate wells
Natural and recombinant mouse interleukin-6 (IL-6)
30X Wash Buffer, 50mL
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Intra-assay CV <10%; inter-assay CV <10%
1 plate kit
Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
No significant cross-reaction with mouse GM-CSF, IFN-g, IL-1aa, IL-2, IL-3, IL-4, IL-5, IL-7, IL-9, IL-10, IL-12, or human IL-6. This kit has been shown to have a high level of cross-reactivity with recombinant rat IL-6
4 hr.
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