Invitrogen™ Mouse GM-CSF Carrier-Free

Recombinant Protein

Brand:  Affymetrix eBioscience 34-8331-85

Product Code. 15548786

Description

GM-CSF (Granulocyte-Macrophage colony-stimulating factor) is a 14.6kDa hematopoietic growth factor that exists in glycosylated and non-glycosylated biologically active forms, and stimulates the development of granulocytes, macrophages, early megakaryocytes and eosinophil progenitor cells. The active form of the GM-CSF protein is found extracellularly as a homodimer and the GM-CSF gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q-syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. The ability of recombinant GM-CSF to increase hematopoietic cell recovery has become a focus area in the therapeutic treatment of patients following bone marrow transplantation. Recent studies have investigated GM-CSF in inflammatory and autoimmune diseases such as arthritis, arthritis related interstitial lung disease, nephritis, and psoriasis. In the CNS, GM-CSF depletion or neutralization has been studied in experimental autoimmune encephalomyelitis (EAE) and multiple sclerosis (MS). Description: Mouse Granulocyte/Macrophage Colony Stimulating Factor (GM-CSF) is an ∽14 kDa factor produced mainly by activated T cells and macrophages. Other cell types, such as endothelium and fibroblasts, also secrete GM-CSF in response to TNF alpha, IL-2, IL-1, and IFN gamma. GM-CSF stimulates growth of macrophages, granulocytes and dendritic cells. GM-CSF is found as a membrane-bound form and also as a complex associated with the extracellular matrix. Non-glycosylated GM-CSF is biologically active. Applications Reported: Mouse GM-CSF Recombinant Protein Carrier-Free has been reported for use in cytokine bioassays. Applications Tested: The ED50 of this protein, as measured by MC/9 cell proliferation assay, is less than or equal to 650 pg/mL. This corresponds to a specific activity of greater than or equal to 1.5 x 10e6 Units/mg. Source: E. coli expressed mature mouse GM-CSF accession &Num; NM_009969. Bioactivity: The ED50 of this protein, as measured by MC/9 cell proliferation assay, is less than or equal to 650 pg/mL. This corresponds to a specific activity of greater than or equal to 1.5 x 10e6 Units/mg. Endotoxin: Less than 0.01ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE. Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.

Specifications

• Accession Number: P01587
• For Use With (Application): Control, Functional Assay
• Gene ID (Entrez): 12981
• Name: Mouse GM-CSF Carrier-Free
• Purification Method: SDS-PAGE
• Source: E. coli
• Regulatory Status: RUO
• Endotoxin Concentration: Less than 0.01ng/μg cytokine as determined by the LAL assay.
• Gene Symbol: Csf2
• Product Type: Recombinant Protein, Carrier-Free
• Conjugate: Unconjugated
• Species: E. coli
• Form: Liquid
• Purity or Quality Grade: Affinity chromatography

• Concentration: 0.5mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Molecular Weight (g/mol): 14kDa
• pH Range: 7.2
• Quantity: 500μg
• Storage Requirements: -80°C
• Structural Form: E. coli expressed mature mouse GM-CSF accession # NM_009969
• Common Name: Mouse GM-CSF Carrier-Free
• Biological Activity: The ED50 of this protein, as measured by MC/9 cell proliferation assay, is less than or equal to 650pg/mL. This corresponds to a specific activity of greater than or equal to 1.5 x 10⁶ Units/mg.
• Specific Reactivity: Mouse
• Cross Reactivity: Ms
• Recombinant: Recombinant
• Protein Subtype: Recombinant