eBioscience™ Mouse TSLP, Recombinant Protein, Carrier-Free

Mouse Recombinant Protein

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 34-8498-85

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Product Code. 15548806

Quantity Price
1 £ 1607.3899999999999 / 500µg
EU Stock 2
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Description and Specification

Specification

Recombinant Yes
Regulatory Status RUO
Biological Activity The ED50 tested in a Nag8/7 cell proliferation assay was 0.15ng/mL, corresponding to a specific activity of approximately 6 x 106 Units/mg.
Gene Symbol Thymic Stromal-Derived Lymphopoietin
Accession Number NM_021367
Cross Reactivity Mouse
Formulation Sterile liquid; 20mM NaH2PO4, 0.15M NaCl, pH 7.2. 0.22μm filtered.
Purification Method SDS-PAGE
Endotoxin Concentration Less than 0.1ng/μg cytokine as determined by the LAL assay.
Molecular Weight 15-22kDa on SDS-PAGE due to glycosylation.
For Use With (Application) Cytokine Bioassays
Name TSLP
Storage Requirements Store at less than or equal to -70°C.
Conjugate Unlabeled
Purity >99%
Quantity 500μg
Structural Form Insect cell expressed N-terminal His-Tagged amino acids Tyr20-Glu140 of mTSLP accession #NM_021367
Product Type Recombinant Protein, Carrier-Free
Source Insect Cells

Thymic Stromal Lymphopoietin (TSLP) is a member of the B cell-stimulating factor family. Originally identified from the conditioned medium of a mouse thymic stromal cell line that promoted B-cell development, TSLP is expressed in thymus, spleen, kidney, lung, and bone marrow. TSLP activity overlaps with that of IL-7, playing a key role in the development of B cells, as well as stimulating the growth of thymocytes and T cells. TSLP stimulates monocytes to produce T cell-attracting chemokines such as TARC (CCL17) and MDC (CCL22). TSLP also activates dendritic cell-primed CD4 T cells to produce Th2 cytokines, including IL-4, IL-5, IL-13, and TNF alpha. This hematopoietic cytokine binds to a heterodimeric receptor complex consisting of the IL-7R alpha chain (CD127) and the TSLP-specific chain (TSLPR). Binding induces activation of STAT3 and STAT5 via phosphorylation. Mouse TSLP shares approximately 43% amino acid sequence identity with human TSLP. Recombinant mouse TSLP is a 15.0 kDa protein consisting of 121 amino acid residues.