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Invitrogen™ eBioscience™ Human Wnt-1, Recombinant Protein, Carrier-Free

Human Recombinant Protein

Brand:  Invitrogen™ 34-8695-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15518826

  • £696.40 / 100µg


Estimated Shipment date
from Supplier 24-11-2017
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Description

Description

Wnt proteins constitute a family of secreted glyocoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal. The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade. Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor. Extensive research has shown that deregulation of these pathways or its components can lead to cancer.

Human Wnt-1 is encoded as a 370 amino acid precursor that, upon secretion, is cleaved into a 343 amino acid (42 kDa) mature form. At the amino acid level, mature human Wnt-1 is approximately 99% identical to mature mouse Wnt-1. Depending on the tissue, Wnt-1 can activate the canonical and non-canonical signaling pathways. Studies have demonstrated its involvement in a variety of functions, including embryonic development and carcinogenesis. Wnt-1 signaling can also induce the phosphorylation and subsequent degradation of c-Myb.
Specifications

Specifications

Functional Assays
42kDa (mature form)
SDS-PAGE and HPLC
100μg
Store lyophilized protein at less than or equal to -20°C. Reconstituted protein solution can be stored with carrier-protein (e.g., 0.1% BSA) at less than or equal to -20°C.
E. coli (amino acids Ala28-Leu370; Accession # NP_005421)
The ED50 of this protein, as determined by human BMP-2 (200ng/mL) induction of alkaline phosphatase production by murine ATDC5 cells, is 1.5-2.5ng/mL.
Unlabeled
Yes
Lyophilized without additive.
Wnt-1
>98%
E. coli
RUO
Less than 0.1ng/μg cytokine as determined by the LAL assay
Recombinant Protein, Carrier-Free
Human