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Mouse IL-33 Carrier-Free, Recombinant Protein, eBioscience™

Recombinant Protein

Brand:  Affymetrix eBioscience 34-8332-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15558786

  • £727.59 / 100µg


EU Stock 3
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Description

Description

IL-33 is a 270 amino acid highly divergent protein belonging to the IL-1 family with an IL-1-like C-terminal domain. It is a dual function protein that may function both as a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. IL-33 binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6. IL-33 activates NF-kappaB and MAP kinases, and drives production of TH2-associated cytokines from in vitro polarized TH2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs. It is proteolytically converted to a mature form by CASP1 and is highly expressed in high endothelial venules found in tonsils, Peyer's patches and mesenteric lymph nodes and is almost undetectable in placenta. Description: Mouse IL-33, also called NF-HEV and DVS 27, is a 30kDa proinflammatory cytokine produced by endothelial and epithelial cells. IL-33 is released during necrotic cell death but was first found in the nucleus of endothelial cells. This dual pattern of expression is reminiscent of ""alarmins"" also known as endogeneous danger signals. Other known alarmins are IL-1 alpha; and HMGB1. IL-33 has been identified as the ligand for ST2 (a member of the Il-1 receptor family). ST2 is stably expressed on mast cells and T(h)2 effector T cells and is functionally associated with T(h)2-mediated inflammation. Although IL-33 was initially reported to be processed by Caspase-1, recent data indicate that Caspase-3 or -7 are capable of processing IL-33 at more physiologic concentrations. IL-33 plays an immune regulatory role by inducing IL-5 and IL-13 in vitro and in vivo and activating basophils, eosinophils and mast cells. In addition to its role in proimflammation, it may also decrease inflammation through interactions with IL-1 thereby blocking its effect. Mouse IL-33 share 55% amino acid sequence identity with human and 90% with rat. Applications Reported: Mouse IL-33 Recombinant Protein Carrier-Free has been reported for use in cytokine bioassays. Applications Tested: This recombinant mouse IL-33 has been tested by bioassays using the cell line D10.G4.1. The ED50 measured in the D10.G4.1 proliferation assay is typically less than 0.1ng/ml, corresponding to a specific activity of greater than 1x107 Units/mg. Source: E. coli expressed protein consisting of amino acids Ser 109-Ile 266 of mature mouse IL-33 accession # AK075849. Bioactivity: Measured by D10.G4.1 cell proliferation assay. The ED50 is 0.03ng/mL, corresponding to a specific activity of 3.0 x 107 Units/mg. Endotoxin: Less than 0.01ng/ug cytokine as determined by the LAL assay. Purity: >97% as determined by SDS-PAGE. Molecular Weight: The DTT reduced protein migrates as a 15kDa polypeptide on SDS-PAGE. Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.
Specifications

Specifications

Q8BVZ5
Cytokine Bioassays
PBS with 2mM TCEP and no preservative; pH 7.2
The DTT reduced protein migrates as a 15kDa polypeptide on SDS-PAGE.
7.2
Affinity chromatography
E. coli
RUO
Recombinant
Mouse IL-33 Carrier-Free
Control, Functional Assay
Recombinant Protein, Carrier-Free
Unlabeled
E. coli
0.5mg/mL
Liquid
77125
Mouse IL-33 Carrier-Free
SDS-PAGE
100μg
-80°C
E. coli expressed protein consisting of amino acids Ser 109-Ile 266 of mature mouse IL-33 accession # AK075849
Less than 0.01ng/μg cytokine as determined by the LAL assay.
Il33
Measured by D10.G4.1 cell proliferation assay. The ED50 is 0.03ng/mL, corresponding to a specific activity of 3.0 x 107 Units/mg.
Mouse
Ms
Recombinant
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