Contains the necessary reagents for performing enzyme linked immunosorbent spot (ELISPOT) assays for high resolution frequency analysis of IL-4-secreting cells. This ELISPOT reagent set is pre-titrated for optimal spot development.
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Contains Sodium azide
For Research Use Only
Includes: Capture Antibody: Pre-titrated, Functional Grade (low endotoxin) purified antibody Detection Antibody. Pre-titrated, biotin-conjugated antibody ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISPOT Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISPOT Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20 and filter with 0.22 uM). Assay Diluent. 5X concentrated Detection enzyme. Pre-titrated Avidin-HRP Certificate of Analysis. Lot-specific instructions for dilution of antibodies and enzyme
The complete, ready-to-use set includes all the reagents needed to perform ELISPOT assays, including: capture and detection antibody, coating buffer powder, assay diluent and detection enzyme. It is supplied with a certificate of anaylsis.