Thermo Scientific™ Pierce™ Mouse IL-4 ELISA Kits

Measure levels of mouse interleukin-4 in mouse serum and culture supernatant samples using 96-well plates.

Overview
Brand: Thermo Scientific™ Pierce™

Manufacturer Part Number: EMIL45

MOUSE IL-4 ELISA

UNSPSC: 41116126

Code: PN

Additional Details:
Additional Details: Weight: 0.10000kg



Product Code. 10783485

Quantity Price
1 £ 1268.0 / 1 set
Estimated Shipment date
from Supplier 23-12-2016
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Description and Specification

Specification

Assay Range 15 to 375pg/mL
Description Mouse IL-4 ELISA Kit
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Formulation Complete ELISA kit with coated 96-well plates
Kit Contents 30X Wash Buffer, 200mL
Precision Intra-assay CV <10%; inter-assay CV <10%
Measurement Absorbance at 450nm minus absorbance at 550nm
Quantity 5 plate kit
Sensitivity <5pg/mL
Sample Type Cell culture supernatant, serum
Sample Size 50┬ÁL
Specificity No significant cross-reaction with with mouse IL-2, IL-3, IL-5, IL-6, IL-7, IFNg, GM-CSF, TNFa; rat IL-4; rabbit IL-4 or human IL-4
Sufficient For 5 x 96 microplate wells
Total Assay Time 3.5 hr.
Target Natural and recombinant mouse interleukin-4 (IL-4)

These mouse IL-4 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-4 (IL-4) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. The antibody-pair kit consists of the matched IL-4 antibody pairs and IL-4 standard only. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant mouse interleukin-4 (IL-4)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 15 to 375pg/mL

  • Sensitivity: < 5pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum

  • Total Assay Time: 3.5 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with with mouse IL-2, IL-3, IL-5, IL-6, IL-7, IFNγ, GM-CSF, TNFα; rat IL-4; rabbit IL-4 or human IL-4

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the antibody-pair kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).

Documentation

Technical documents