eBioscience™ Annexin V, Recombinant Protein

Recombinant Protein

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: BMS306/30UG

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Product Code. 15581907

Quantity Price
1 £ 92.96 / 30µg
Estimated Shipment date
from Supplier 04-05-2017
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Description and Specification

Specification

Name Annexin V
For Use With (Application) Flow Cytometric Analysis
Molecular Weight 35.8kDa
Gene Symbol Lipocortin V, Endonexin II, Calphobindin I
Recombinant Yes
Source E. coli
Biological Activity Annexin V exhibits anti-phospholipase activity and binds to phosphatidylserine (PS).
Quantity 30μg
Regulatory Status RUO
Formulation Lyophilized, reconstitution in 1mL distilled water (incubate at 30°C for 5 minutes). Reconstituted solution contains 20mM phosphate buffer (pH 7.0), 0.02% Tween 80, 130mM Arginine HCL.
Storage Requirements The lyophilized protein is stable at 2-8deg;C as indicated on the label. Reconstituted Annexin V should be stored in working aliquots at -20°C.
Purification Method SDS-gel electrophoresis and reverse phase HPLC
Conjugate Unlabeled
Purity >89%
Product Type Recombinant Protein
Structural Form E. Coli

Annexins are a family of calcium-dependent phospholipid-binding proteins, which bindto phosphatidylserine (PS) to identify apoptotic cells. In healthy cells, PS is predominantlylocated along the cytosolic side of the plasma membrane. Upon initiation of apoptosis,PS loses its asymmetric distribution in the phospholipid bilayer and translocates tothe extracellular membrane, which is detectable with fluorescently labeled AnnexinV. In early stages of apoptosis, the plasma membrane excludes viability dyes such aspropidium iodide (PI) and 7-AAD, therefore cells which display only Annexin V staining(PI/7-AAD negative) are in early stages of apoptosis. During late-stage apoptosis, loss ofcell membrane integrity allows Annexin V binding to cytosolic PS, as well as cell uptakeof PI and 7-AAD. Annexin V staining, paired with 7-AAD or PI is widely used to identifyapoptotic stages by flow cytometry.