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Invitrogen™ eBioscience™ Annexin V, Recombinant Protein

Recombinant Protein

Brand:  Invitrogen™ BMS306-300UG

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15571907

  • £465.04 / 300µg

EU Stock 1
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Annexins are a family of calcium-dependent phospholipid-binding proteins, which bindto phosphatidylserine (PS) to identify apoptotic cells. In healthy cells, PS is predominantlylocated along the cytosolic side of the plasma membrane. Upon initiation of apoptosis,PS loses its asymmetric distribution in the phospholipid bilayer and translocates tothe extracellular membrane, which is detectable with fluorescently labeled AnnexinV. In early stages of apoptosis, the plasma membrane excludes viability dyes such aspropidium iodide (PI) and 7-AAD, therefore cells which display only Annexin V staining(PI/7-AAD negative) are in early stages of apoptosis. During late-stage apoptosis, loss ofcell membrane integrity allows Annexin V binding to cytosolic PS, as well as cell uptakeof PI and 7-AAD. Annexin V staining, paired with 7-AAD or PI is widely used to identifyapoptotic stages by flow cytometry.


Flow Cytometric Analysis
SDS-gel electrophoresis and reverse phase HPLC
The lyophilized protein is stable at 2-8deg;C as indicated on the label. Reconstituted Annexin V should be stored in working aliquots at -20°C.
E. Coli
Annexin V exhibits anti-phospholipase activity and binds to phosphatidylserine (PS).
Lyophilized, reconstitution in 1mL distilled water (incubate at 30°C for 5 minutes). Reconstituted solution contains 20mM phosphate buffer (pH 7.0), 0.02% Tween 80, 130mM Arginine HCL.
Annexin V
E. coli
Lipocortin V, Endonexin II, Calphobindin I
Recombinant Protein