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Mouse TSLP Carrier-Free, Recombinant Protein, eBioscience™

Recombinant Protein

Brand:  Affymetrix eBioscience 34-8498-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15538806

  • £731.55 / 100µg


Estimated Shipment date
from Supplier 30-07-2018
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Description

Description

TSLP is a 159 amino acid four helix-bundle hemopoietic cytokine that signals through a heterodimeric receptor complex consisting of the human TSLP receptor and the IL-7R alpha-chain. It impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes, enhances the maturation of CD11c (+) dendritic cells, naive CD4 (+), and CD8 (+) T cells into proallergic effectors, and primes dendritic cells to produce large amounts of IL-12 after CD40 ligand stimulation. TSLP influences the regulation of the positive selection of regulatory T cells and maintenance of peripheral CD4 (+) T cell homeostasis. It induces allergic inflammation by directly activating mast cells and may be involved in the pathophysiology of inflammatory arthritis. It is expressed in heart, liver and prostate. Description: Thymic Stromal Lymphopoietin (TSLP) is a member of the B cell-stimulating factor family. Originally identified from the conditioned medium of a mouse thymic stromal cell line that promoted B-cell development, TSLP is expressed in thymus, spleen, kidney, lung, and bone marrow. TSLP activity overlaps with that of IL-7, playing a key role in the development of B cells, as well as stimulating the growth of thymocytes and T cells. TSLP stimulates monocytes to produce T cell-attracting chemokines such as TARC (CCL17) and MDC (CCL22). TSLP also activates dendritic cell-primed CD4 T cells to produce Th2 cytokines, including IL-4, IL-5, IL-13, and TNF alpha. This hematopoietic cytokine binds to a heterodimeric receptor complex consisting of the IL-7R alpha chain (CD127) and the TSLP-specific chain (TSLPR). Binding induces activation of STAT3 and STAT5 via phosphorylation. Mouse TSLP shares approximately 43% amino acid sequence identity with human TSLP. Recombinant mouse TSLP is a 15.0kDa protein consisting of 121 amino acid residues. Applications Reported: Recombinant mouse TSLP is biologically active. Applications Tested: This recombinant mouse TSLP has been tested in a Nag8/7 cell proliferation assay with an observed ED50 of 0.15ng/mL, corresponding to a specific activity of approximately 6x10e6 Units/mg. Source: Insect cell expressed N-terminal His-Tagged amino acids Tyr20-Glu140 of mTSLP accession #NM_021367. Bioactivity: The ED50 tested in a Nag8/7 cell proliferation assay was 0.15ng/mL, corresponding to a specific activity of approximately 6 x 10e6 Units/mg. Endotoxin: Less than 0.1ng/ug cytokine as determined by the LAL assay. Purity: >99% as determined by SDS-PAGE. Molecular Weight: 15-22kDa on SDS-PAGE due to glycosylation. Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.
Specifications

Specifications

Q9JIE6
Cytokine Bioassays
PBS with no preservative; pH 7.2
15-22kDa on SDS-PAGE due to glycosylation.
7.2
Affinity chromatography
Insect Cells
RUO
Recombinant
Mouse TSLP Carrier-Free
Control, Functional Assay
Recombinant Protein, Carrier-Free
Unlabeled
Insect cells
0.5mg/mL
Liquid
53603
Mouse TSLP Carrier-Free
SDS-PAGE
100μg
-80°C
Insect cell expressed N-terminal His-Tagged amino acids Tyr20-Glu140 of mTSLP accession #NM_021367
Less than 0.1ng/μg cytokine as determined by the LAL assay.
Tslp
The ED50 tested in a Nag8/7 cell proliferation assay was 0.15ng/mL, corresponding to a specific activity of approximately 6 x 106 Units/mg.
Mouse
Ms
Recombinant
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