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Invitrogen™ IL-28 Mouse ELISA Kit

For the quantitative detection of mouse IL-28.

Brand:  Invitrogen™ BMS6028TEN

1600.08 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15591997

  • £2400.00 / Pack of 10
Estimated Shipment: 22-04-2024
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Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IL-28
Biotin-Conjugate anti-mouse IL-28 monoclonal antibody
Streptavidin-HRP
Mouse IL-28 Standard lyophilized, 4000pg/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x

Description

Description

The Mouse Interleukin-28A (Ms IL-28A) ELISA quantitates Ms IL-28A in mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-28A. Principle of the method The Mouse IL-28A solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL-28A belongs to the IFN lambda family, a novel family of cytokines within the IL-10 superfamily. The three members of this family are IL-29 (IFN lambda 1), IL-28A (IFN lambda 2), and IL-28B (IFN lambda 3), and are also known as the type III Interferons.The IFN lambdas signal through a heterodimeric receptor of which one subunit, IL-10R2, is shared with other members of the superfamily. The second subunit, IFN lambda R1 or IL-28R alpha, is unique to the IFN lambdas. Signaling occurs through the Jak/STAT pathway in a similar manner as the type I IFN (IFN alpha/beta) and activates many of the same genes despite low sequence homology between the cytokines and receptors in the two families. Both IFN families display antiviral activity through the induction of antiviral protein production in target cells and the upregulation of MHC class I expression. These proteins also exhibit antiproliferative and antitumor effects, making them a possible alternative to IFN alpha cancer therapies. Unlike the type I IFN, which are able to stimulate most cells, response to IFN lambda stimulation appears to be limited to dendritic and some tumor cells due to the limited expression of IFN lambda R1. Another notable difference is the ability of the IFN lambda stimulation to drive dendritic cells towards the production of CD4+CD25+FoxP3+ regulatory T cells, suggesting a possible immunoregulatory role.
Specifications

Specifications

Q4VK74
12.85 pg/mL
ELISA Kit
Mouse
Colorimetric Microplate Reader
EG330496,IL-28A,Il28a
5.6%
HRP
RUO
2°C to 8°C
Mouse
3 hr. 30 min.
31.3-2,000 pg/mL
Biotin
Plasma, Serum, Supernatant
ELISA
330496
7.2%
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
10 x 96 Tests
Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
IFNL2, IFNL3 Interleukin 28, IL-28A, IL-28B
1 hr. 20 min.
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