eBioscience™ Mouse LIF, Recombinant Protein, Carrier-Free

Mouse Recombinant Protein

Brand: eBioscience™

Manufacturer Part Number: 34-8521-82

100UG Mouse LIF Recombinant Protein Carrier-Free

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg

Product Code. 15598806

Quantity Price
1 £614.00 / 100µg
Estimated Shipment date
from Supplier 28-10-2016
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Description and Specification


Accession Number NM_008501
Concentration 0.5mg/mL
Conjugate Unlabeled
Cross Reactivity Mouse
For Use With (Application) Cytokine Bioassays
Formulation Sterile liquid: phosphate buffered saline, 0.05% Tween-20, 0.22μm filtered
Molecular Weight 20kDa
Name LIF
Purification Method SDS-PAGE
Purity >98%
Quantity 100μg
Recombinant Yes
Source E. coli
Storage Requirements Store at less than or equal to -70°C.
Regulatory Status RUO
Structural Form E. coli derived Ser24-Phe203 accession number NM_008501
Endotoxin Concentration Less than 0.01ng/μg cytokine as determined by the LAL assay
Gene Symbol leukocyte inhibitory factor, Leukemia inhibitory factor
Biological Activity Recombinant mouse LIF has been tested for induction of mIL-6 in the M1 cell line. The ED50 in this assay is 0.75-0.4ng/mL, which corresponds to a specific activity of 1.3 x 106 - 2.5 x 106 Units/mg. When used at 10ng/mL, this recombinant protein has also been found to support mouse embryonic stem cells in an undifferentiated state at same concentration.
Product Type Recombinant Protein, Carrier-Free

Leukemia inhibitory factor (LIF) is a 20 kDa protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF can be up-regulated by pro-inflammatory cytokines such as TNFα and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal.Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific.