Invitrogen™ Mouse IL-23 (Carrier-Free) Recombinant Protein, eBioscience™

Recombinant Protein

Brand:  Invitrogen™ 34-8231-82

Product Code. 15598766

Description

IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN-γ. Mouse IL-23 does induce strong proliferation of memory T cells (but not naive T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-γ production by naive and memory T cells, as compared to IL-12. Description: IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN-gamma. Mouse IL-23 does induce strong proliferation of memory T cells (but not naive T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-gamma production by naive and memory T cells, as compared to IL-12. Applications Reported: Mouse IL-23 Recombinant Protein Carrier-Free has been reported for use in cytokine bioassays. Applications Tested: The ED50 of this protein, as measured by IL-17A induction in mouse splenocytes, is less than or equal to 625 pg/mL. This corresponds to a specific activity of greater than 1.5 x 10e6 Units/mg. Source: Insect cells infected with baculovirus: mouse p40, amino acids met 1-Ser 355, accession &Num; NM_008352 was co-expressed with mouse p19, amino acids met 1-Ala 196, accession &Num; AF301619. Bioactivity: The ED50 of this protein, as measured by IL-17A induction in mouse splenocytes, is less than or equal to 625 pg/mL. This corresponds to a specific activity of greater than 1.5 x 10e6 Units/mg. Endotoxin: Less than 0.01ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE. Molecular Weight: The heterodimer of p40, amino acids met 23-ser 355, cystine-linked to p19, amino acids leu 20-ala 196, has a predicted molecular mass of 55,616. On non-reducing SDS-PAGE the heterodimeric cystine-linked protein migrates as a 60 kDa protein. The DTT reduced protein migrates as 43 kDa and 21 kDa polypeptides. Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.

Specifications

• Accession Number: Q9EQ14
• For Use With (Application): Control, Functional Assay
• Gene ID (Entrez): 83430
• Name: Mouse IL-23 Carrier-Free
• Purification Method: SDS-PAGE
• Source: Insect Cells
• Regulatory Status: RUO
• Endotoxin Concentration: Less than 0.01ng/μg cytokine as determined by the LAL assay.
• Gene Symbol: Il23a
• Product Type: Recombinant Protein, Carrier-Free
• Conjugate: Unconjugated
• Species: Baculovirus
• Form: Liquid
• Purity or Quality Grade: Affinity chromatography

• Concentration: 0.5mg/mL
• Formulation: PBS with no preservative; pH 6.0
• Molecular Weight (g/mol): The heterodimer of p40, amino acids met 23-ser 355, cystine-linked to p19, amino acids leu 20-ala 196, has a predicted molecular mass of 55,616. On non-reducing SDS-PAGE the heterodimeric cystine-linked protein migrates as a 60kDa protein. The DTT reduced protein migrates as 43kDa and 21kDa polypeptides.
• pH Range: 6.0
• Quantity: 100μg
• Storage Requirements: -80°C
• Structural Form: Insect cells infected with baculovirus: mouse p40, amino acids met 1-ser 355, accession # NM_008352 was co-expressed with mouse p19, amino acids met 1-ala 196, accession # AF301619
• Common Name: Mouse IL-23 Carrier-Free
• Biological Activity: The ED50 of this protein, as measured by IL-17A induction in mouse splenocytes, is less than or equal to 625pg/mL. This corresponds to a specific activity of greater than 1.5 x 10⁶ Units/mg.
• Specific Reactivity: Mouse
• Cross Reactivity: Ms
• Recombinant: Recombinant
• Protein Subtype: Recombinant